基于胶原蛋白/生理凝结纤维蛋白的纳米生物支架，负载纳米银：一种新方法。
Collagen/physiologically clotted fibrin-based nanobioscaffold supported with silver nanoparticles: A novel approach.
机构信息
Engineering Faculty, Leather Engineering Department, Ege University, Izmir, Turkey.
School of Bio & Chemical Engineering, Sathyabama University, Chennai, Tamil Nadu, India.
出版信息
Int J Artif Organs. 2022 Dec;45(12):1021-1027. doi: 10.1177/03913988221119529. Epub 2022 Aug 22.
PURPOSE
In this work, a blend of collagen, physiologically clotted fibrin (PCF), and silver nanoparticles (AgNPs) is used to develop a nanobioscaffold (NBS), for their possible application in wound dressing materials.
METHODS
The prepared NBS were evaluated using physicochemical, mechanical, and antibacterial properties. The NBS cell viability was demonstrated in a biocompatibility study using the human keratinocyte cell line (HaCaT).
RESULTS
The results demonstrated that the NBS had excellent tensile strength (22.15 ± 0.05 MPa), elongation at break (13.32 ± 0.09%), and water absorption (97.51 ± 0.08). The in-vitro study demonstrated its biocompatible nature. NBS exhibited significant antibacterial activity against the Gram-negative and Gram-positive bacteria.
CONCLUSION
The NBS with required mechanical strength, antibacterial activity, and biocompatibility may be tested as a wound material in rats after getting the necessary approval.
目的
本工作中，将胶原蛋白、生理凝结纤维蛋白（PCF）和银纳米粒子（AgNPs）混合，开发出一种纳米生物支架（NBS），以期将其应用于伤口敷料材料。
方法
使用物理化学、机械和抗菌性能对制备的 NBS 进行评估。使用人角质形成细胞系（HaCaT）进行的生物相容性研究证明了 NBS 的细胞活力。
结果
结果表明，NBS 具有优异的拉伸强度（22.15±0.05 MPa）、断裂伸长率（13.32±0.09%）和吸水率（97.51±0.08%）。体外研究表明其具有生物相容性。NBS 对革兰氏阴性菌和革兰氏阳性菌均表现出显著的抗菌活性。
结论
NBS 具有所需的机械强度、抗菌活性和生物相容性，在获得必要的批准后，可作为大鼠伤口敷料进行测试。
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