College of Pharmacy, Inje University, 197 Inje-ro, Gimhae, Gyeongnam, 621-749, Republic of Korea.
National Creative Research Initiatives (NCRI) Center for Isogeometric Optimal Design, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 151-744, Republic of Korea.
Arch Pharm Res. 2016 Apr;39(4):465-473. doi: 10.1007/s12272-016-0718-8. Epub 2016 Feb 19.
The search for novel antibacterial agents is necessary to combat microbial resistance to current antibiotics. Silver nanoparticles (AgNPs) have been reported to be effective antibacterial agents. Tannic acid is a polyphenol compound from plants with antioxidant and antibacterial activities. In this report, AgNPs were prepared from silver ions by tannic acid-mediated green synthesis (TA-AgNPs). The reaction process was facile and involved mixing both silver ions and tannic acid. The absorbance at 423 nm in the UV-Visible spectra demonstrated that tannic acid underwent a reduction reaction to produce TA-AgNPs from silver ions. The synthetic yield of TA-AgNPs was 90.5% based on inductively coupled plasma mass spectrometry analysis. High-resolution transmission electron microscopy and atomic force microscopy images indicated that spherical-shaped TA-AgNPs with a mean particle size of 27.7-46.7 nm were obtained. Powder high-resolution X-ray diffraction analysis indicated that the TA-AgNP structure was face-centered cubic with a zeta potential of -27.56 mV. The hydroxyl functional groups of tannic acid contributed to the synthesis of TA-AgNPs, which was confirmed by Fourier transform infrared spectroscopy. The in vitro antibacterial activity was measured using the minimum inhibitory concentration (MIC) method. The TA-AgNPs were more effective against Gram-negative bacteria than Gram-positive bacteria. The MIC for the TA-AgNPs in all of the tested strains was in a silver concentration range of 6.74-13.48 μg/mL. The tannic acid-mediated synthesis of AgNPs afforded biocompatible nanocomposites for antibacterial applications.
新型抗菌剂的寻找对于应对当前抗生素的微生物耐药性是必要的。纳米银颗粒（AgNPs）已被报道为有效的抗菌剂。鞣酸是一种来自植物的多酚化合物，具有抗氧化和抗菌活性。在本报告中，AgNPs 是通过鞣酸介导的绿色合成（TA-AgNPs）从银离子制备的。反应过程简单，涉及混合银离子和鞣酸。紫外可见光谱中 423nm 处的吸光度表明，鞣酸发生还原反应，从银离子生成 TA-AgNPs。基于电感耦合等离子体质谱分析，TA-AgNPs 的合成产率为 90.5%。高分辨率透射电子显微镜和原子力显微镜图像表明，获得了具有 27.7-46.7nm 平均粒径的球形 TA-AgNPs。粉末高分辨率 X 射线衍射分析表明 TA-AgNP 结构为面心立方，zeta 电位为-27.56mV。鞣酸的羟基官能团有助于 TA-AgNPs 的合成，这通过傅里叶变换红外光谱得到了证实。使用最低抑菌浓度（MIC）法测量了体外抗菌活性。TA-AgNPs 对革兰氏阴性菌的抑制效果优于革兰氏阳性菌。在所有测试菌株中，TA-AgNPs 的 MIC 在银浓度范围为 6.74-13.48μg/mL。鞣酸介导的 AgNPs 合成提供了用于抗菌应用的生物相容的纳米复合材料。
