Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province 710072, China.
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, Shaanxi Province 710072, China; College of Enology, Northwest A&F University, YangLing, Shaanxi Province, China.
Int J Biol Macromol. 2021 Jun 30;181:1183-1195. doi: 10.1016/j.ijbiomac.2021.04.119. Epub 2021 Apr 21.
Silver nanoparticles (AgNPs) are widely used in wound dressing, but are limited in the application due to its high toxicity at effective concentrations. iturin-AgNPs was previously found to have much higher antibacterial activity at lower AgNPs content than the commercial AgNPs. To verify its potential application in the promotion of wound healing, a chitosan (CS) composite sponge dressing-loaded iturin-AgNPs was developed and evaluated for their antibacterial activity in vitro and used for wound healing in vivo. As results, the synthesized CS dressing had high porosity and water absorption. As expected, the antibacterial activity of CS dressing was significantly promoted by the incorporation of iturin-AgNPs. The CS dressing-loaded iturin-AgNPs showed more effective inhibition of bacterial infection and promotion of wound healing processing and quality than the commercial wound dressing loaded AgNPs in vivo. The mechanisms for the promotion of wound healing by the CS dressing-loaded iturin-AgNPs were found as the enhancement of re-epithelialization and collagen formation, as well as the increased antibacerial activity. No toxicity was found to all organs of mice. The study developed an efficient way to enhance the antibacterial activity of CS dressing loaded AgNPs at low toxicity, which has great potential in wound care application.
银纳米粒子（AgNPs）广泛应用于伤口敷料，但由于其在有效浓度下的高毒性，其应用受到限制。先前发现，与商业 AgNPs 相比，伊枯草素-AgNPs 在较低的 AgNPs 含量下具有更高的抗菌活性。为了验证其在促进伤口愈合方面的潜在应用，开发了一种壳聚糖（CS）复合海绵敷料负载伊枯草素-AgNPs，并对其体外抗菌活性进行了评价，并用于体内伤口愈合研究。结果表明，合成的 CS 敷料具有较高的孔隙率和吸水性。正如预期的那样，伊枯草素-AgNPs 的掺入显著促进了 CS 敷料的抗菌活性。与负载 AgNPs 的商业伤口敷料相比，CS 敷料负载伊枯草素-AgNPs 更有效地抑制了细菌感染，并促进了伤口愈合过程和质量。CS 敷料负载伊枯草素-AgNPs 促进伤口愈合的机制被发现是增强了再上皮化和胶原形成，以及增加了抗菌活性。在所有小鼠器官中均未发现毒性。该研究开发了一种有效提高负载 AgNPs 的 CS 敷料抗菌活性的方法，在低毒性下具有很大的伤口护理应用潜力。
