School of Environmental & Rural Science, University of New England, Armidale, New South Wales, Australia.
Hanwoo Research Institute, National Institute of Animal Science, RDA, Pyeongchang, Korea.
Anim Genet. 2022 Dec;53(6):863-866. doi: 10.1111/age.13251. Epub 2022 Aug 22.
The aim of this study was to find significant genomic regions associated with carcass traits in Hanwoo cattle and to compare the benefit of using additional information from non-genotyped animals. Imputed whole-genome sequence data were used along with phenotypic data on 13 715 genotyped animals as well as phenotypes of 440 284 non-genotyped animals that were offspring of 454 genotyped sires. For carcass weight, 15 083 SNPs in 33 QTL regions and 313 candidate genes were identified. We found 410 SNPs in 17 QTL regions containing 122 candidate genes for back fat thickness. In total, 656 SNPs in 19 QTLs with 137 candidate genes for eye muscle area and 79 SNPs in 12 QTL regions with 77 candidate genes were identified for marbling score. The most important candidate genes included ZFAT, TG, PLAG1, CHCHD7, and TOX for carcass weight and eye muscle area, NOG for back fat thickness, and EVOVL5 for marbling score. This study showed that the use of phenotypic records on non-genotyped progeny along with imputed whole-genome sequence data increased the power of detecting new significant genomic regions.
本研究旨在寻找与韩牛胴体性状相关的重要基因组区域，并比较利用未基因型动物的额外信息的益处。我们使用了全基因组序列数据的估计值，以及 13715 头基因型动物的表型数据，以及 454 头基因型公牛的 440284 头未基因型后代的表型数据。对于胴体重量，在 33 个 QTL 区域和 313 个候选基因中共鉴定出 15083 个 SNP。我们在包含 122 个候选基因的 17 个 QTL 区域中发现了 410 个 SNP，用于背脂厚度。总共在包含 137 个候选基因的 19 个 QTL 区域中鉴定出 656 个 SNP，用于眼肌面积，在包含 77 个候选基因的 12 个 QTL 区域中鉴定出 79 个 SNP，用于大理石花纹评分。最重要的候选基因包括 ZFAT、TG、PLAG1、CHCHD7 和 TOX 用于胴体重量和眼肌面积，NOG 用于背脂厚度，EVOVL5 用于大理石花纹评分。本研究表明，利用未基因型后代的表型记录以及全基因组序列数据的估计值增加了检测新的重要基因组区域的能力。
