Division of Animal & Dairy Science, Chungnam National University, Daejeon 34134, Republic of Korea.
Department of Bio-AI Convergence, Chungnam National University, Daejeon 34134, Republic of Korea.
Genes (Basel). 2023 Sep 22;14(10):1839. doi: 10.3390/genes14101839.
This study aimed to identify causal variants associated with important carcass traits such as weight and meat quality in Hanwoo cattle. We analyzed missense mutations extracted from imputed sequence data () and performed an exon-specific association test on the carcass traits of 16,970 commercial Hanwoo. We found 33, 2, 1, and 3 significant SNPs associated with carcass weight (CW), backfat thickness (BFT), eye muscle area (EMA), and marbling score (MS), respectively. In CW and EMA, the most significant missense SNP was identified at 19,524,263 on BTA14 and involved the . A missense SNP in the , located at 107,160,304 on BTA2 was identified as being involved in BFT. For MS, missense SNP in the gene, located at 11,849,704 in BTA22 was identified as the most significant marker. The contribution of the most significant missense SNPs to genetic variance was confirmed to be 8.47%, 2.08%, 1.73%, and 1.19% in CW, BFT, EMA, and MS, respectively. We generated favorable and unfavorable haplotype combinations based on the significant SNPs for CW. Significant differences in GEBV (Genomic Estimated Breeding Values) were observed between groups with each favorable and unfavorable haplotype combination. In particular, the missense SNPs in , , and appear to significantly affect the protein's function and structure, making them strong candidates as causal mutations. These missense SNPs have the potential to serve as valuable markers for improving carcass traits in Hanwoo commercial farms.
本研究旨在鉴定与韩牛重要胴体性状（如体重和肉质）相关的因果变异。我们分析了从已推测序列数据中提取的错义突变，并对 16970 头商业韩牛的胴体性状进行了外显子特异性关联测试。我们发现了 33 个、2 个、1 个和 3 个与胴体重量（CW）、背膘厚度（BFT）、眼肌面积（EMA）和大理石花纹评分（MS）显著相关的 SNP。在 CW 和 EMA 中，最显著的错义 SNP 位于 BTA14 上的 19524263 位，涉及 。在 BTA2 上位于 107160304 位的 基因中的一个错义 SNP 被鉴定为与 BFT 相关。对于 MS，在 BTA22 上位于 11849704 位的 基因中的错义 SNP 被鉴定为最显著的标记。最显著错义 SNP 对 CW、BFT、EMA 和 MS 遗传方差的贡献分别被确认为 8.47%、2.08%、1.73%和 1.19%。我们根据对 CW 有显著影响的 SNP 生成了有利和不利的单倍型组合。在具有每个有利和不利单倍型组合的组之间观察到 GEBV（基因组估计育种值）的显著差异。特别是 、 和 中的错义 SNP 似乎显著影响蛋白质的功能和结构，使其成为强候选因果突变。这些错义 SNP 有可能成为改善韩牛商业农场胴体性状的有价值的标记。
