Institute of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Institute of Chemistry, Baghdad-ul-Jadeed Campus, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan.
Int J Biol Macromol. 2022 Nov 1;220:788-801. doi: 10.1016/j.ijbiomac.2022.08.119. Epub 2022 Aug 20.
Chitosan (CS)/metal oxide (MO) nano-carriers have recently attracted attention due to their great integration into several biomedical applications. Herein, CS and dysprosium oxide based bio-nanocomposites (DyO/CuFeO/CS) were prepared using a citrate sol-gel route for biomedical settings at large and drug delivery, in particular. The chemical structure, average crystallite size, and surface morphology of DyO/CuFeO/CS bio-nanocomposites were characterized using spectroscopic techniques, including FT-IR, PXRD, and SEM. The prepared nano composite's drug loading or release kinetics were investigated by FT-IR, zeta potential (ZP), and ultraviolet-visible spectroscopy (UV-Vis). In the FT-IR spectrum, the peaks in the range of 800-400 cm confirmed the formation of meta-oxides, while amide bands at 1661 and 1638 cm revealed the existence of CS in the bio-nanocomposite. The peaks at 2θ = 35.46 and 28.5, 39.4 indicated the presence and chemical interaction of DyO and CuFeO respectively. The crystallite size was <20 nm. The model drug used in the loading and in vitro release assays was ciprofloxacin hydrochloride. Ciprofloxacin's CF stretch caused a modest peak to be seen at 1082 cm and changed in zeta potential value from 7.90 mV to 8.88 mV endorsing that the drug had been loaded onto the nanomaterial. The loading efficiency (%) of CIP onto the composite was from 25 to 30 %, calculated from optical density measurements. Different kinetic models, such as zero-order, first-order, Higuchi, Hixon-Crowell, and Korsmeyer-Peppas, were determined to confirm the drug release mechanism. The percent (%) of drug release from the surface of DyO/CuFeO/CS in PBS (pH 7.4), acidic (pH 2.2) and basic (pH 9.4) dissolution media were found to be 70, 28 and 20 %, respectively. Drug kinetics showed that mainly the release is fickian type followed "Fick's law of diffusion", slightly deviated from fickian release (dissolution-dependent system). Korsmeyer-Peppas (R 0.9773, n < 0.4) and Higuchi's (R 0.9846) models were the best for fitting controlled drug release data. The results revealed that the DyO/CuFeO/CS bio-nanocomposite has good potential for a controlled drug delivery system.
壳聚糖（CS）/金属氧化物（MO）纳米载体由于其在多种生物医学应用中的出色整合而受到关注。本文采用柠檬酸盐溶胶-凝胶法制备了 CS 和氧化镝基生物纳米复合材料（DyO/CuFeO/CS），用于生物医学领域的大型应用和药物输送，特别是。采用光谱技术（包括 FT-IR、PXRD 和 SEM）对 DyO/CuFeO/CS 生物纳米复合材料的化学结构、平均晶粒尺寸和表面形态进行了表征。通过 FT-IR、zeta 电位（ZP）和紫外-可见光谱（UV-Vis）研究了纳米复合材料的药物负载或释放动力学。在 FT-IR 光谱中，800-400 cm 范围内的峰证实了金属氧化物的形成，而酰胺带在 1661 和 1638 cm 处表明 CS 存在于生物纳米复合材料中。2θ=35.46 和 28.5、39.4 处的峰分别表明 DyO 和 CuFeO 的存在和化学相互作用。晶粒尺寸<20 nm。在负载和体外释放试验中使用的模型药物是盐酸环丙沙星。环丙沙星的 CF 伸展导致在 1082 cm 处出现一个适度的峰，并导致 zeta 电位值从 7.90 mV 变为 8.88 mV，这表明药物已负载到纳米材料上。根据光密度测量，从复合材料上负载 CIP 的效率（%）为 25%至 30%。通过确定零阶、一阶、Higuchi、Hixon-Crowell 和 Korsmeyer-Peppas 等不同的动力学模型，确定了药物释放机制。在 PBS（pH 7.4）、酸性（pH 2.2）和碱性（pH 9.4）溶解介质中，DyO/CuFeO/CS 表面的药物释放百分比（%）分别为 70%、28%和 20%。药物动力学表明，主要的释放是菲克型，遵循“菲克扩散定律”，略微偏离菲克型释放（溶解依赖性系统）。Korsmeyer-Peppas（R 0.9773，n<0.4）和 Higuchi 模型（R 0.9846）最适合拟合控释药物数据。结果表明，DyO/CuFeO/CS 生物纳米复合材料具有作为控释药物输送系统的良好潜力。
