在兔半月板缺损模型中使用脂肪源性干细胞片进行再生可改善12周时半月板的拉伸强度和负荷分布功能。

Regeneration Using Adipose-Derived Stem Cell Sheets in a Rabbit Meniscal Defect Model Improves Tensile Strength and Load Distribution Function of the Meniscus at 12 Weeks.

作者信息

Kimura Mitsuhiro, Nakase Junsuke, Takata Yasushi, Shimozaki Kengo, Asai Kazuki, Yoshimizu Rikuto, Kanayama Tomoyuki, Yanatori Yusuke, Tsuchiya Hiroyuki

机构信息

Department of Orthopedic Surgery, Graduate School of Medical Science, Kanazawa University, Kanazawa, Japan.

出版信息

Arthroscopy. 2023 Feb;39(2):360-370. doi: 10.1016/j.arthro.2022.07.023. Epub 2022 Aug 19.

DOI:10.1016/j.arthro.2022.07.023

PMID:35995333

Abstract

PURPOSE

The purpose of this study was to evaluate the mechanical properties, such as the tensile strength and load distribution function, of the meniscus tissue regenerated using adipose-derived stem cell (ADSC) sheets in a rabbit meniscal defect model.

METHODS

ADSC sheets were prepared from adipose tissue of rabbits. The anterior half of the medial meniscus was removed from both knees. One knee was transplanted with an ADSC sheet; the contralateral knee was closed without transplantation. Mechanical tests were performed at 4 and 12 weeks posttransplantation. In the tensile test, tensile force was applied to the entire medial meniscus, including the normal area (n = 10/group). Compression tests were performed on the entire knee, with soft tissues other than the ligament removed. A pressure-sensitive film was inserted under the medial meniscus and a 40-N load was applied (n = 5/group).

RESULTS

In the tensile test, the elastic modulus in ADSC-treated knees was higher at 12 weeks (ADSC: 70.30 ± 18.50 MPa, control: 43.71 ± 7.11 MPa, P = .009). The ultimate tensile strength (UTS) in ADSC-treated knees at 12 weeks was also higher (ADSC: 22.69 ± 5.87 N, control: 15.45 ± 4.08 N, P = .038). In the compression test, the contact area was larger in the ADSC group at 4 weeks (ADSC: 31.60 ± 8.17 mm, control: 20.33 ± 2.86 mm, P = .024) and 12 weeks (ADSC: 41.07 ± 6.09 mm, control: 30.53 ± 5.47 mm, P = .04). Peak pressure was significantly lower in ADSC-treated knees at 12 weeks (ADSC: 11.91 ± 1.03 MPa, control: 15.53 ± 2.3 MPa, P = .002).

CONCLUSIONS

The regenerated meniscus tissue, 12 weeks after transplantation of the ADSC sheets into the meniscal defect area, had high elastic modulus and UTS. In the meniscus-tibia compartment, the contact area was large and the peak pressure was low.

CLINICAL RELEVANCE

ADSC sheets promoted regeneration of meniscus. ADSC sheet transplantation for meniscal defects could be an effective regenerative therapy.

摘要

目的

本研究旨在评估在兔半月板缺损模型中，使用脂肪来源干细胞（ADSC）片再生的半月板组织的力学性能，如拉伸强度和负荷分布功能。

方法

从兔脂肪组织制备ADSC片。从双膝关节切除内侧半月板的前半部分。一侧膝关节移植ADSC片；对侧膝关节不进行移植直接闭合。在移植后4周和12周进行力学测试。在拉伸试验中，对包括正常区域在内的整个内侧半月板施加拉力（每组n = 10）。对整个膝关节进行压缩试验，去除韧带以外的软组织。在内侧半月板下方插入压敏膜并施加40 N的负荷（每组n = 5）。

结果

在拉伸试验中，ADSC治疗组膝关节在12周时的弹性模量更高（ADSC：70.30±18.50 MPa，对照组：43.71±7.11 MPa，P = .009）。ADSC治疗组膝关节在12周时的极限拉伸强度（UTS）也更高（ADSC：22.69±5.87 N，对照组：15.45±4.08 N，P = .038）。在压缩试验中，ADSC组在4周（ADSC：31.60±8.17 mm，对照组：20.33±2.86 mm，P = .024）和12周（ADSC：41.07±6.09 mm，对照组：30.53±5.47 mm，P = .04）时的接触面积更大。ADSC治疗组膝关节在12周时的峰值压力显著更低（ADSC：11.91±1.03 MPa，对照组：15.53±2.3 MPa，P = .002）。