痰聚合酶链反应在痰非结核分枝杆菌培养阴性的囊性纤维化患者中检测非结核分枝杆菌的价值
The Value of Sputum Polymerase Chain Reaction for Detection of Nontuberculous Mycobacteria in Cystic Fibrosis Patients with Negative Nontuberculous Mycobacteria Sputum Culture.
作者信息
Mirlohi Seyed Hossein, Eftekhari Kambiz, Shirzadi Rohola, Fateh Abolfazl, Masoumi Morteza, Modaresi Mohammadreza
机构信息
Pediatric Respiratory and Sleep Medicine Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
Pediatric Gastroenterology and Hepatology Research Center, Department of Pediatrics, Bahrami Children's Hospital, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Med J Islam Repub Iran. 2022 Feb 12;36:7. doi: 10.47176/mjiri.36.7. eCollection 2022.
Cystic Fibrosis (CF) is a life-threatening autosomal recessive disease. The purpose of this study was to evaluate the value of Polymerase Chain Reaction (PCR) in CF patients with Nontuberculous Mycobacteria (NTM) negative sputum culture. This is a descriptive cross-sectional study. The population included all children with CF, aged between 5 - 18 years old, with an NTM negative sputum culture. The patient's sputum samples were sent for smear and culture of NTM, RFLP PCR, and PCR sequence. In total, 57 CF patients with negative NTM sputum culture were enrolled. Nine patients (15.78%) had positive sputum PCR for NTM. Among these strains, Mycobacterium simiae was the most common one with 5 cases (8.77% of total positive cases). PCR can be used as an alternative diagnostic method for NTM in CF patients with negative NTM sputum culture, always under clinical suspicion of the disease.
囊性纤维化（CF）是一种危及生命的常染色体隐性疾病。本研究的目的是评估聚合酶链反应（PCR）在非结核分枝杆菌（NTM）痰培养阴性的CF患者中的价值。这是一项描述性横断面研究。研究对象包括所有年龄在5至18岁之间、NTM痰培养阴性的CF儿童。患者的痰标本被送去进行NTM涂片和培养、限制性片段长度多态性PCR以及PCR测序。总共纳入了57例NTM痰培养阴性的CF患者。9例患者（15.78%）NTM痰PCR检测呈阳性。在这些菌株中，猿猴分枝杆菌最为常见，有5例（占总阳性病例的8.77%）。在临床始终怀疑患有该疾病的情况下，PCR可作为NTM痰培养阴性的CF患者NTM的替代诊断方法。
Zotero 插件