King’s College London, UK.
Clin Microbiol Infect. 2010 Nov;16(11):1656-8. doi: 10.1111/j.1469-0691.2010.03189.x.
The DNA-based techniques used to detect bacteria in clinical samples are unable to discriminate between live bacteria, dead bacteria, and extracellular DNA. This failure to limit analysis to viable bacterial cells represents a significant problem, leading to false-positive results, as well as a failure to resolve the impact of antimicrobial therapy. The use of propidium monoazide treatment significantly reduces the contribution of dead cells and extracellular DNA to such culture-independent analyses. Here, the increased ability to resolve the impact of antibiotic therapy on Pseudomonas aeruginosa load in cystic fibrosis respiratory samples reveals statistically significant changes that would otherwise go undetected.
用于检测临床样本中细菌的基于 DNA 的技术无法区分活细菌、死细菌和细胞外 DNA。这种无法将分析仅限于存活细菌细胞的方法存在严重问题，会导致假阳性结果，并无法解决抗菌治疗的影响。使用吖啶橙单甲醚处理可显著减少死细胞和细胞外 DNA 对这种非培养依赖性分析的贡献。在这里，能够更准确地评估抗生素治疗对囊性纤维化呼吸道样本中铜绿假单胞菌负荷的影响，揭示了否则会被忽略的具有统计学意义的变化。
