Yang Xinyu, Huang Qifeng, Xu Jitao, Gao Zhen, Jiang Xue, Wu Yuanhua, Ye Wenwu, Liang Yue
College of Plant Protection, Shenyang Agricultural University, Shenyang, Liaoning, China.
College of Plant Protection, Nanjing Agricultural University, Nanjing, Jiangsu, China.
J Appl Microbiol. 2022 Dec;133(6):3585-3595. doi: 10.1111/jam.15792. Epub 2022 Sep 7.
Lovastatin has been indicated to impair growth and development of Phytophthora sojae. Therefore, this study was performed to understand the inhibitory mechanism of lovastatin and investigate the metabolic pathway potentially served as a new control target for this plant pathogen.
Whole transcriptome analysis of lovastatin-treated P. sojae was performed by RNA-sequencing. The results revealed that 84 genes were upregulated and 58 were downregulated with more than fourfold changes under treatment. Kyoto Encyclopaedia of Genes and Genomes analysis indicated that the branched-chain amino acids (BCAAs) biosynthesis pathway was abundantly enriched. All enzymes in the BCAAs biosynthesis pathway were identified in the P. sojae genome. Moreover, the study found that the herbicide flumetsulam targeting acetohydroxyacid synthase (AHAS) of the BCAAs biosynthesis pathway could effectively inhibit mycelial growth of P. sojae.
Lovastatin treatment significantly influences the BCAAs biosynthesis pathway in P. sojae. Moreover, the herbicide flumetsulam targets AHAS and inhibits growth of P. sojae.
The present study revealed that BCAAs biosynthesis pathway was influenced by lovastatin treatment and its key enzyme AHAS was identified as a potential new control target, which provides clues for exploring more oomycetes to control plant diseases caused by P. sojae.
已有研究表明洛伐他汀会损害大豆疫霉的生长和发育。因此，本研究旨在了解洛伐他汀的抑制机制，并探究可能作为这种植物病原体新防治靶点的代谢途径。
通过RNA测序对经洛伐他汀处理的大豆疫霉进行全转录组分析。结果显示，处理后有84个基因上调，58个基因下调，变化幅度超过四倍。京都基因与基因组百科全书分析表明，支链氨基酸（BCAAs）生物合成途径显著富集。在大豆疫霉基因组中鉴定出了BCAAs生物合成途径中的所有酶。此外，研究发现靶向BCAAs生物合成途径乙酰羟酸合酶（AHAS）的除草剂氟嘧磺隆可有效抑制大豆疫霉的菌丝生长。
洛伐他汀处理显著影响大豆疫霉的BCAAs生物合成途径。此外，除草剂氟嘧磺隆靶向AHAS并抑制大豆疫霉的生长。
本研究揭示了BCAAs生物合成途径受洛伐他汀处理的影响，并确定其关键酶AHAS为潜在的新防治靶点，为探索更多防治大豆疫霉引起的植物病害的卵菌提供了线索。
