Integrated Molecular Plant Physiology Research (IMPRES); Biology Department, University of Antwerp, Antwerp, Belgium.
Plant Biochemistry & Biotechnology Lab, Department of Agriculture, Hellenic Mediterranean University, Heraklion, Greece.
Plant Signal Behav. 2022 Dec 31;17(1):2104002. doi: 10.1080/15592324.2022.2104002.
Over the years, many different growth media have been used to grow in petri dishes. For these media the nutrient composition may vary, sugars may or may not be added, the medium may or may not be buffered and there is a choice between different gelling agents. The magnitude of possible combinations of these variables obstructs easy comparison of seedling phenotypes grown on the different media. This is especially obvious when it concerns the study of root hairs that are extremely sensitive to changes in their environment. To demonstrate this effect, we have grown wild-type seeds on 18 different combinations of growth media and quantified root hair development. Comparison of root hair length and the respective root hair profiles identified the media that result in the formation of the longest root hairs. On these favored media they elongate through tip growth at a constant growth rate until they reach their final length (around 0.6 mm) at a distance of ±4 mm from the root tip.
多年来，人们一直在培养皿中使用许多不同的生长培养基来培养 。这些培养基的营养成分可能有所不同，可能添加也可能不添加糖，培养基可能有缓冲也可能没有缓冲，并且可以在不同的凝胶剂之间进行选择。这些变量的可能组合的数量之多，阻碍了在不同培养基上生长的幼苗表型的容易比较。当涉及到对极其敏感的根毛的研究时，这种情况尤为明显。为了证明这种效果，我们在 18 种不同的生长培养基组合上培养了 野生型种子，并对根毛发育进行了定量分析。比较根毛长度和相应的根毛图谱确定了导致形成最长根毛的培养基。在这些有利的培养基上，它们通过尖端生长以恒定的生长速率伸长，直到它们在距根尖 ±4mm 的距离处达到最终长度（约 0.6mm）。
