Yonkov D, Georgiev V, Kambourova T, Opitz M
Methods Find Exp Clin Pharmacol. 1987 Apr;9(4):205-8.
The experiments were carried out on male albino rats trained and tested for retention (24 hr later) in a shuttle-box. Angiotensin II (AT II) 0.10 micrograms intracerebroventricularly (i.c.v.), gamma-aminobutyric acid (GABA) 100 micrograms i.c.v., bicuculline 0.5 and 1.0 mg/kg intraperitoneally (i.p.), and picrotoxin 0.5 and 1.0 mg/kg i.p. administered independently or in combinations immediately after training. AT II was found to improve retention. GABA also facilitated retention. Combination of AT II + GABA potentiated the memory-improving effect of AT II. Bicuculline and picrotoxin at a dose of 0.5 mg/kg did not affect retention, while at a dose of 1.0 mg/kg they improved it. Combinations of AT II + bicuculline (0.5 mg/kg) and AT II + picrotoxin (0.5 mg/kg) abolished the retention-improving effect of AT II. Bicuculline (0.5 mg/kg) or picrotoxin (0.5 mg/kg) abolished the retention-facilitating effect of the combination of AT II + GABA as well as the potentiating action of GABA on the memory effect of AT II. These results suggest the participation of GABAergic transmission in the CNS in the mechanisms of the long-term memory-improving effect of AT II.
实验选用雄性白化大鼠，在穿梭箱中进行训练并于24小时后测试记忆保持情况。训练后立即分别或联合给予脑室内注射0.10微克血管紧张素II（AT II）、脑室内注射100微克γ-氨基丁酸（GABA）、腹腔注射0.5和1.0毫克/千克荷包牡丹碱以及腹腔注射0.5和1.0毫克/千克印防己毒素。结果发现，AT II可改善记忆保持情况。GABA也有助于记忆保持。AT II与GABA联合使用可增强AT II改善记忆的效果。0.5毫克/千克剂量的荷包牡丹碱和印防己毒素不影响记忆保持，而1.0毫克/千克剂量则可改善记忆保持。AT II与0.5毫克/千克荷包牡丹碱以及AT II与0.5毫克/千克印防己毒素联合使用时，AT II改善记忆保持的效果消失。0.5毫克/千克荷包牡丹碱或0.5毫克/千克印防己毒素可消除AT II与GABA联合使用时促进记忆保持的效果以及GABA对AT II记忆效果的增强作用。这些结果表明，中枢神经系统中GABA能传递参与了AT II改善长期记忆效果的机制。
