Department of Oncology and Hematology, The Second Hospital of Jilin University, Changchun, Jilin, China.
Crit Rev Eukaryot Gene Expr. 2022;32(7):25-33. doi: 10.1615/CritRevEukaryotGeneExpr.2022043160.
The microRNA (miRNA) miR-576-5p was reported to facilitate tumor progression, but its underlying regulatory impacts on colon adenocarcinoma remain unknown. This work therefore attempted to examine the biological effects of miR-576-5p in colon adenocarcinoma. The Cancer Genome Atlas (TCGA) database was introduced to analyze miR-576-5p and NEGR1 messenger RNA (mRNA) expression levels between normal and cancer tissues. miR-576-5p and NEGR1 expression levels in colon adenocarcinoma cells and colon cells were evaluated with quantitative reverse transcription polymerase chain reaction (qRT-PCR). NEGR1 protein expression was examined by Western blot. Furthermore, colon adenocarcinoma cell behaviors were evaluated via CCK-8, wound-healing, Transwell, and hanging drop experiments. The interaction between miR-576-5p and NEGRI was verified by dual-luciferase assay. miR-576-5p was upregulated in colon adenocarcinoma, and miR-576-5p overexpression notably facilitated the proliferative, migratory, invasive abilities of colon adenocarcinoma cells. NEGR1 was newly identified as one target of miR-576-5p, and, miR-576-5p/NEGR1 axis was subsequently verified to modulate cell proliferative, migratory, invasive, and aggregate abilities. miR-576-5p facilitated aggressive progression of colon adenocarcinoma cells by targeting NEGR1, which could be an underlying therapeutic target for colon adenocarcinoma.
微小 RNA（miRNA）miR-576-5p 被报道可促进肿瘤进展，但它对结肠腺癌的潜在调节作用尚不清楚。因此，本研究试图研究 miR-576-5p 在结肠腺癌中的生物学效应。本研究引入癌症基因组图谱（TCGA）数据库分析正常组织和肿瘤组织中 miR-576-5p 和 NEGR1 信使 RNA（mRNA）的表达水平。采用实时定量逆转录聚合酶链反应（qRT-PCR）检测结肠腺癌细胞和结肠细胞中 miR-576-5p 和 NEGR1 的表达水平。采用 Western blot 检测 NEGR1 蛋白表达水平。进一步通过 CCK-8、划痕愈合、Transwell 和悬滴实验评估结肠腺癌细胞行为。采用双荧光素酶报告基因实验验证 miR-576-5p 和 NEGR1 之间的相互作用。miR-576-5p 在结肠腺癌中上调，miR-576-5p 过表达显著促进结肠腺癌细胞的增殖、迁移和侵袭能力。NEGR1 被新鉴定为 miR-576-5p 的一个靶基因，miR-576-5p/NEGR1 轴随后被证实可调节细胞增殖、迁移、侵袭和聚集能力。miR-576-5p 通过靶向 NEGR1 促进结肠腺癌细胞的侵袭性进展，这可能是结肠腺癌的一个潜在治疗靶点。
