National Institute for Laser, Plasma and Radiation Physics (INFLPR), Atomiştilor Street 409, 077125 Măgurele, Ilfov, Romania.
Faculty of Physics, Research and Development Center for Materials and Electronic & Optoelectronic Devices (MDEO), University of Bucharest, Atomiştilor Street 405, 077125 Măgurele, Ilfov, Romania.
Biosensors (Basel). 2022 Jul 27;12(8):573. doi: 10.3390/bios12080573.
Environmental pollution with cadmium (Cd) is a major concern worldwide, with prolonged exposure to this toxic heavy metal causing serious health problems, such as kidney damage, cancer, or cardiovascular diseases, only to mention a few. Herein, a gold-coated reflection-type fiber optic--surface plasmon resonance (Au-coated FO-SPR) sensor is manufactured and functionalized with (i) bovine serum albumin (BSA), (ii) chitosan, and (iii) polyaniline (PANI), respectively, for the sensitive detection of cadmium ions (Cd2+) in water. Then, the three sensor functionalization strategies are evaluated and compared one at a time. Out of these strategies, the BSA-functionalized FO-SPR sensor is found to be highly sensitive, exhibiting a limit of detection (LOD) for Cd2+ detection at nM level. Moreover, the presence of Cd2+ on the FO-SPR sensor surface was confirmed by the X-ray photoelectron spectroscopy (XPS) technique and also quantified consecutively for all the above-mentioned functionalization strategies. Hence, the BSA-functionalized FO-SPR sensor is sensitive, provides a rapid detection time, and is cheap and portable, with potential applicability for monitoring trace-level amounts of Cd within environmental or potable water.
环境污染与镉（Cd）是全世界关注的主要问题，长期接触这种有毒重金属会导致严重的健康问题，如肾脏损害、癌症或心血管疾病等，仅举几例。在此，制造了一种金涂层反射式光纤-表面等离子体共振（Au-coated FO-SPR）传感器，并分别用（i）牛血清白蛋白（BSA）、（ii）壳聚糖和（iii）聚苯胺（PANI）进行功能化，用于水中镉离子（Cd2+）的灵敏检测。然后，依次评估和比较这三种传感器功能化策略。在这些策略中，发现 BSA 功能化的 FO-SPR 传感器具有高度灵敏性，对 Cd2+检测的检测限（LOD）达到纳摩尔级。此外，通过 X 射线光电子能谱（XPS）技术证实了 FO-SPR 传感器表面存在 Cd2+，并连续对所有上述功能化策略进行了定量。因此，BSA 功能化的 FO-SPR 传感器具有灵敏度高、检测时间快、成本低、便携等优点，具有在环境或饮用水中监测痕量 Cd 的潜在应用价值。
