全内反射椭圆偏振法在布洛克表面波生物传感应用中的研究。

Total Internal Reflection Ellipsometry Approach for Bloch Surface Waves Biosensing Applications.

机构信息

State Research Institute Center for Physical Sciences and Technology, Saulėtekio Ave. 3, LT-10257 Vilnius, Lithuania.

Faculty of Electronics, Vilnius Gediminas Technical University, Naugarduko St. 41, LT-03227 Vilnius, Lithuania.

出版信息

Biosensors (Basel). 2022 Jul 30;12(8):584. doi: 10.3390/bios12080584.

DOI:10.3390/bios12080584

PMID:36004980

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9405594/

Abstract

A one-dimensional photonic crystal with an additional TiO layer, supporting Bloch surface waves (BSW), was used for enhanced signal sensitivity for the detection of protein interaction. To compare the optical response of BSW and photonic crystals (PC), bovine serum albumin and specific antibodies against bovine serum were used as a model system. The results obtained show the enhanced sensitivity of p- and s-BSW components for the 1D PC sample with an additional TiO layer. Furthermore, a higher sensitivity was obtained for the BSW component of p-polarization in the PC sample with an additional TiO layer, where the sensitivity of the ellipsometric parameter Ψ was five times higher and that of the Δ parameter was eight times higher than those of the PC sample. The capabilities of BSW excitations are discussed from the sensitivity point of view and from the design of advanced biosensing.

摘要

具有附加 TiO 层的一维光子晶体，支持 Bloch 表面波（BSW），用于增强信号灵敏度，以检测蛋白质相互作用。为了比较 BSW 和光子晶体（PC）的光学响应，使用牛血清白蛋白和针对牛血清的特异性抗体作为模型系统。得到的结果表明，具有附加 TiO 层的一维 PC 样品中 p- 和 s-BSW 分量的灵敏度得到了增强。此外，在具有附加 TiO 层的 PC 样品中，p 偏振的 BSW 分量获得了更高的灵敏度，其中椭圆参数 Ψ 的灵敏度高 5 倍，Δ 参数的灵敏度高 8 倍比 PC 样品高。从灵敏度的角度和先进生物传感的设计出发，讨论了 BSW 激发的功能。