Huang Cheng, Wang Dianwen, Chen Hongping, Deng Wei, Chen Dazhou, Chen Ping, Wang Jilin
Rice National Engineering Research Center (Nanchang), Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China.
Curr Issues Mol Biol. 2022 Jul 27;44(8):3351-3363. doi: 10.3390/cimb44080231.
The DUF26 domain-containing protein is an extracellular structural protein, which plays an important role in signal transduction. Dongxiang wild rice ( Griff.) is the northern-most common wild rice in China. Using domain analysis, 85 DUF26 domain-containing genes were identified in Dongxiang wild rice (DXWR) and further divided into four categories. The DUF26 domain-containing genes were unevenly distributed on chromosomes, and there were 18 pairs of tandem repeats. Gene sequence analysis showed that there were significant differences in the gene structure and motif distribution of the DUF26 domain in different categories. Motifs 3, 8, 9, 13, 14, 16, and 18 were highly conserved in all categories. It was also found that there were eight plasmodesmata localization proteins (PDLPs) with a unique motif 19. Collinearity analysis showed that DXWR had a large number of orthologous genes with wheat, maize, sorghum and zizania, of which 17 DUF26 domain-containing genes were conserved in five gramineous crops. Under the stress of anaerobic germination and seedling submergence treatment, 33 DUF26 domain-containing genes were differentially expressed in varying degrees. Further correlation analysis with the expression of known submergence tolerance genes showed that these DUF26 domain-containing genes may jointly regulate the submergence tolerance process with these known submergence tolerance genes in DXWR.
含有DUF26结构域的蛋白是一种细胞外结构蛋白，在信号转导中起重要作用。东乡野生稻（Griff.）是中国分布最北的普通野生稻。通过结构域分析，在东乡野生稻（DXWR）中鉴定出85个含有DUF26结构域的基因，并进一步分为四类。含有DUF26结构域的基因在染色体上分布不均，有18对串联重复。基因序列分析表明，不同类别中DUF26结构域的基因结构和基序分布存在显著差异。基序3、8、9、13、14、16和18在所有类别中高度保守。还发现有8个具有独特基序19的胞间连丝定位蛋白（PDLP）。共线性分析表明，DXWR与小麦、玉米、高粱和菰有大量直系同源基因，其中17个含有DUF26结构域的基因在5种禾本科作物中保守。在厌氧萌发和幼苗淹水处理胁迫下，33个含有DUF26结构域的基因有不同程度的差异表达。进一步与已知耐淹基因的表达进行相关性分析表明，这些含有DUF26结构域的基因可能与DXWR中这些已知耐淹基因共同调控耐淹过程。
