Quaresma Ana Judith Pires Garcia, Rodrigues Yan Corrêa, Aboim Joseline Barbosa, Bezerra Mayza Miranda, Gouveia Maria Isabel Montoril, Da Costa Ana Roberta Fusco, de Oliveira Souza Cintya, Bastos Flávia Corrêa, Lima Luana Nepomuceno Gondim Costa, de Paula Ramos Francisco Lúzio, Valéria Batista Lima Karla
Bacteriology and Mycology Section, Evandro Chagas Institute (SABMI/IEC), Health Surveillance Secretariat, Ministry of Health, Ananindeua 67030-000, PA, Brazil.
Ph.D. Program in Parasitic Biology in the Amazon Region (PPGBPA), State University of Pará (UEPA), Tv. Perebebuí, 2623-Marco, Belém 66087-662, PA, Brazil.
Infect Dis Rep. 2022 Aug 3;14(4):569-573. doi: 10.3390/idr14040060.
Typhoidal salmonellosis is a global public health problem occurring in developing endemic regions. In Brazil, cases are mostly registered in the North and Northeast regions. Molecular characterization of the strains is important to understand the epidemiology of disease infections and to design control strategies. The present study retrospectively evaluates the genotyping features of sporadic and outbreak-related Typhi isolates from the Brazilian North region. Bacterial isolates were recovered from blood and a rectal swab of patients in the states of Acre and Pará, Brazilian North region, in the period of 1995 to 2013, and were submitted to genotyping by applying Multilocus sequence typing (MLST) and Pulsed Field Gel Electrophoresis (PFGE) reference methods. MLST genotyping revealed the presence of epidemic clones ST1 and ST2, and 20 pulsotypes were identified by PFGE, including four distinct clusters (A-D), and six subclusters (A1-D1) with indistinguishable strains in different periods and locations. To conclude, the obtained data demonstrates the temporal stability, adaptation, and transmission of outbreak-related and sporadic Typhi strains over time, contributing to the transmission chain in the region.
伤寒型沙门氏菌病是发展中流行地区出现的一个全球公共卫生问题。在巴西，病例大多登记在北部和东北部地区。菌株的分子特征对于了解疾病感染的流行病学以及设计控制策略很重要。本研究回顾性评估了巴西北部地区散发性和与暴发相关的伤寒杆菌分离株的基因分型特征。1995年至2013年期间，从巴西北部地区阿克里州和帕拉州患者的血液和直肠拭子中分离出细菌分离株，并通过应用多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）参考方法进行基因分型。MLST基因分型显示存在流行克隆ST1和ST2，PFGE鉴定出20种脉冲型，包括四个不同的簇（A - D）和六个亚簇（A1 - D1），不同时期和地点的菌株难以区分。总之，所获得的数据证明了与暴发相关的和散发性伤寒杆菌菌株随时间的时间稳定性、适应性和传播，这对该地区的传播链有影响。
