Wu Shuang-Xiong, Chen Yang, Lei Quan, Peng Yuan-Yuan, Jiang Hong-Bo
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400715, China.
International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Insects. 2022 Aug 11;13(8):721. doi: 10.3390/insects13080721.
The oriental fruit fly, , is one of the most destructive fruit insect pests. -cypermethrin has been widely used in the orchard to control this major insect. Based on the resistance monitoring in 2011, developed significant resistance against -cypermethrin in fields. This indicated that the has been exposed to sublethal concentrations of -cypermethrin in the field for a long time. Thus, it is urgent to understand the sublethal effects of -cypermethrin on this fly to guide the rational use of an insecticide. According to the olfactory preference assays and electroantennogram (EAG) recording, the after -cypermethrin exposure (LD = 10 ng/fly) severely decreased the ability to perceive the tested odorants. Moreover, we then performed quantitative real-time PCR and found the chemosensory genes including odorant receptor co-receptor () and ionotropic receptor co-receptors (BdorIRcos) were obviously suppressed. Our results demonstrated that the sublethal dose of -cypermethrin impairs the olfaction of the pest insects by suppressing the expression of chemosensory genes ( and BdorIRcos), which expanded our knowledge of the sublethal effects of the pesticide on insects.
东方果实蝇是最具破坏性的水果害虫之一。高效氯氰菊酯已在果园中广泛用于防治这种主要害虫。根据2011年的抗性监测，东方果实蝇在田间已对高效氯氰菊酯产生了显著抗性。这表明东方果实蝇在田间长期接触亚致死浓度的高效氯氰菊酯。因此，迫切需要了解高效氯氰菊酯对这种果蝇的亚致死效应，以指导杀虫剂的合理使用。根据嗅觉偏好试验和触角电位（EAG）记录，高效氯氰菊酯暴露（LD = 10 ng/果蝇）后的东方果实蝇感知受试气味的能力严重下降。此外，我们随后进行了定量实时PCR，发现包括气味受体共受体（）和离子型受体共受体（BdorIRcos）在内的化学感受基因明显受到抑制。我们的结果表明，亚致死剂量的高效氯氰菊酯通过抑制化学感受基因（和BdorIRcos）的表达损害害虫的嗅觉，这扩展了我们对农药对昆虫亚致死效应的认识。
