Yang Yang, Xiong Ying, Li Hong-Fei, Zhao Huai-Jia, Tang Guang-Hui, Meng Li-Wei, Wang Jin-Jun, Jiang Hong-Bo
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, China; International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, China; State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University, China; International Joint Laboratory of China-Belgium on Sustainable Crop Pest Control, Academy of Agricultural Sciences, Southwest University, Chongqing 400716, China.
Pestic Biochem Physiol. 2021 May;174:104808. doi: 10.1016/j.pestbp.2021.104808. Epub 2021 Feb 25.
The neuropeptide adipokinetic hormone (AKH) binds to the AKH receptor (AKHR) to regulate carbohydrate and lipid metabolism. It also participates in the insect anti-stress response. We used RT-qPCR to detect the expression levels of 39 neuropeptides in malathion-susceptible (MS) and malathion-resistant (MR) strains of Bactrocera dorsalis. AKH and AKHR were highly expressed in the MR strain. Using a malathion bioassay and RNA interference (RNAi), we demonstrated that AKHR is involved in the susceptibility of B. dorsalis to malathion. We found significantly reduced expression of two detoxification enzyme genes (glutathione-S-transferase, GST and α-esterase, CarE) after AKHR RNAi. Based on our previous data, GSTd10 and CarE6 participate the direct metabolism of malathion in this fly, which is also verified by a malathion metabolism assay by HPLC using the crude enzymes in the current study. These results suggest that AKHR plays an important role in affecting malathion susceptibility via detoxification enzyme genes.
神经肽促脂肪动激素（AKH）与AKH受体（AKHR）结合，以调节碳水化合物和脂质代谢。它还参与昆虫的抗应激反应。我们使用逆转录定量聚合酶链反应（RT-qPCR）检测了桔小实蝇马拉硫磷敏感（MS）品系和马拉硫磷抗性（MR）品系中39种神经肽的表达水平。AKH和AKHR在MR品系中高表达。通过马拉硫磷生物测定和RNA干扰（RNAi），我们证明了AKHR与桔小实蝇对马拉硫磷的敏感性有关。我们发现AKHR RNA干扰后两种解毒酶基因（谷胱甘肽-S-转移酶，GST和α-酯酶，CarE）的表达显著降低。基于我们之前的数据，GSTd10和CarE6参与了该果蝇对马拉硫磷的直接代谢，本研究通过使用粗酶的高效液相色谱法进行的马拉硫磷代谢测定也证实了这一点。这些结果表明，AKHR通过解毒酶基因在影响马拉硫磷敏感性方面发挥重要作用。
