Bu Ting, Zhang Ming, Lee Sun-Hee, Cheong Yu Eun, Park Yukyung, Kim Kyoung Heon, Kim Dongwon, Kim Sooah
Department of Environment Science & Biotechnology, Jeonju University, Jeonju 55069, Korea.
University Provincial Key Laboratory for Protection and Utilization of Longdong Bio-Resources in Gan-Su Province, College of Life Sciences and Technology, Longdong University, Qingyang 745000, China.
Metabolites. 2022 Aug 3;12(8):717. doi: 10.3390/metabo12080717.
Skin has heterogenous identities on different body sites despite similar cellular compositions. There are two types of skin, volar (palmoplantar) and non-volar (dorsal), which are characterized by epidermal thickness, pigmentation, and presence of hair follicles. However, the mechanisms underlying the development of these different skin types remain unclear. To investigate these, we profiled the cellular metabolites of volar and non-volar skin in mice using gas chromatography-time-of-flight/mass spectrometry (GC-TOF/MS), and further assessed the metabolic differences between them. In total, 96 metabolites from both volar and non-volar skin of mice were identified using the BinBase database system. Metabolomics analysis revealed important differences associated with amino acid metabolism (phenylalanine, tyrosine, and tryptophan biosynthesis; aspartate and glutamate metabolism), sugar metabolism (pentose phosphate pathway), and nucleotide metabolism (pyrimidine metabolism) in volar skin. Fifty metabolites were identified as potential biomarkers differentiating the physiological characteristics of these skin types. Of these, nine were highly increased whereas 41 were significantly decreased in volar skin compared with those in non-volar skin. Overall, these results provide valuable information for understanding the metabolic differences between volar and non-volar skin.
尽管细胞组成相似，但皮肤在身体不同部位具有异质性特征。皮肤有两种类型，掌跖部（手掌和足底）和非掌跖部（背部），其特征在于表皮厚度、色素沉着和毛囊的存在。然而，这些不同皮肤类型发育的潜在机制仍不清楚。为了研究这些机制，我们使用气相色谱 - 飞行时间/质谱（GC - TOF/MS）对小鼠掌跖部和非掌跖部皮肤的细胞代谢物进行了分析，并进一步评估了它们之间的代谢差异。使用BinBase数据库系统总共鉴定出了小鼠掌跖部和非掌跖部皮肤中的96种代谢物。代谢组学分析揭示了掌跖部皮肤中与氨基酸代谢（苯丙氨酸、酪氨酸和色氨酸生物合成；天冬氨酸和谷氨酸代谢）、糖代谢（磷酸戊糖途径）和核苷酸代谢（嘧啶代谢）相关的重要差异。50种代谢物被鉴定为区分这些皮肤类型生理特征的潜在生物标志物。其中，与非掌跖部皮肤相比，掌跖部皮肤中有9种代谢物显著增加，41种代谢物显著减少。总体而言，这些结果为理解掌跖部和非掌跖部皮肤之间的代谢差异提供了有价值的信息。
