Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands.
J Invest Dermatol. 2012 Nov;132(11):2565-72. doi: 10.1038/jid.2012.192. Epub 2012 Jun 14.
The dermis contains two distinct layers: the papillary and the reticular layers. In vitro cultures of the fibroblasts from these layers show that they are different. However, no molecular markers to differentiate between the two subtypes of fibroblasts are known. We performed gene expression analysis on cultured fibroblasts isolated from the papillary and reticular dermis. In all, 116 genes were found to be expressed differentially. Of these, 13 were validated by quantitative reverse transcriptase-PCR analysis and two markers could be validated at the protein level in monolayer cultures. Three markers showed differential expression in in vivo skin sections. The identified, characteristic markers of the two fibroblast subpopulations provide useful tools to perform functional studies on reticular and papillary fibroblasts.
乳头层和网状层。对这些层的成纤维细胞进行体外培养表明它们是不同的。然而，目前还没有分子标记物可以区分两种成纤维细胞亚型。我们对从乳头层和网状真皮分离培养的成纤维细胞进行了基因表达分析。总共发现有 116 个基因表达存在差异。其中，通过定量逆转录聚合酶链反应分析验证了 13 个，并且在单层培养物中可以在蛋白质水平上验证两个标记物。在体内皮肤切片中发现了三个标记物的差异表达。这两种成纤维细胞亚群的特征标记物为研究网状和成纤维细胞的功能提供了有用的工具。
