Lando D Iu, Fridman A S
Mol Biol (Mosk). 1987 Mar-Apr;21(2):330-7.
The influence of the extended interacted under adsorption ligands with a selective binding on the DNA helix-coil transition has been theoretically studied. It was found that contact interaction between ligands or/and their extent give rise to a marked non-linearity of the GC-content dependence of the melting temperature. This non-linearity causes a few features of the dependence of the melting range width on ligand concentration [delta T(C0)]. Such as a non-monotony of the delta T (C0) increase in the presence of ligands increasing the difference between the thermostabilities of poly(d(A-T)] and poly[d(G-C)] polymers. The degree of a non-linearity defines the character of changes of the form of the differential melting curves in the presence of ligands.
理论上研究了具有选择性结合的吸附配体之间扩展相互作用对DNA螺旋-线圈转变的影响。发现配体之间的接触相互作用或/及其程度导致解链温度的GC含量依赖性出现明显的非线性。这种非线性导致解链范围宽度对配体浓度[ΔT（C0）]的依赖性呈现出一些特征。例如，在存在增加聚（d（A-T）]和聚[d（G-C）]聚合物热稳定性差异的配体时，ΔT（C0）增加呈现非单调性。非线性程度决定了存在配体时微分解链曲线形式变化的特征。
