Department of Anaesthesiology and Intensive Medicine, Toxinology Center, First Medical Faculty, Charles University, General University Hospital, U Nemocnice 499/2, 128 08 Prague, Czech Republic.
Department of Biochemistry, Institute of Hematology and Blood Transfusion, U Nemocnice 2094/1, 128 00 Prague, Czech Republic.
Toxins (Basel). 2022 Aug 6;14(8):538. doi: 10.3390/toxins14080538.
Envenomations that are caused by snakebites are mostly accompanied by venom-induced consumption coagulopathy (VICC) with defibrination. The clinical course of VICC is well described; however, reports about its detailed effects in the hemocoagulation systems of patients are sparse. In this pilot study, we prospectively analyzed the changes in plasma fibrinogen that were caused by the envenomation of six patients by five non-European snakes. Western blot analysis was employed and fibrinogen fragments were visualized with the use of specific anti-human fibrinogen antibodies. All of the studied subjects experienced hypo- or afibrinogenemia. The western blot analysis demonstrated fibrinogenolysis of the fibrinogen chains in all of the cases. Fibrinogenolysis was considered to be a predominant cause of defibrination in , , and envenomation; while, in the cases of VICC that were caused by and envenomation, the splitting of the fibrinogen chains was present less significantly.
蛇伤引起的中毒，大多伴有蛇毒诱导的消耗性凝血病（VICC）伴纤维蛋白溶解。VICC 的临床过程已有很好的描述；然而，关于其在患者凝血系统中的详细影响的报告却很少。在这项初步研究中，我们前瞻性地分析了 6 例非欧洲蛇类咬伤 5 例患者引起的血浆纤维蛋白原的变化。采用 Western blot 分析，并使用特异性抗人纤维蛋白原抗体显示纤维蛋白原片段。所有研究对象均出现低纤维蛋白原血症或无纤维蛋白血症。Western blot 分析显示所有病例的纤维蛋白原链纤维蛋白溶解。纤维蛋白原溶解被认为是 、 、 蛇咬伤引起的纤维蛋白溶解的主要原因；而 、 蛇咬伤引起的 VICC 中，纤维蛋白原链的分裂则不太明显。
