Moroshkina E B, Stepanova T F, Raketskaia V V, Frisman E V
Mol Biol (Mosk). 1987 Mar-Apr;21(2):389-95.
The comparative studies of the formation of DNA-complexes with the acridines containing one and two chromophores were accomplished. It was shown that both of acridines were bonded with DNA by means of intercalation irrespective of the ionic strength of medium (mu). When mu = 0.1 the diacridine (1,6-bis(9-acridylamino)-hexan) behaves as an mono-intercalator. Under these conditions both of the ligands exert equal influence of the molecular parameters of DNA. When mu = 0.001 the binding mode of the diacridine with DNA depends on its concentration in a complex. If a number of diacridine molecules on a pair of nucleotides (r) falls in a region 0 less than r less than 0.2 its binding with DNA is accomplished via the bis-intercalation mode and accompanied by the structure distortion of the monomer remnant of the macromolecule. As r increases from 0.2 to 0.4 the gradual change of the binding mode of the diacridine with DNA from bis-intercalation to mono-intercalation takes place. Moreover the structure of nucleotides is reduced. When mu = 0.001 the behaviour of DNA complexes with mono-acridine is analogous to the observed one when mu = 0.1.
完成了对含一个和两个发色团的吖啶与DNA形成复合物的比较研究。结果表明，无论介质的离子强度（μ）如何，两种吖啶均通过嵌入作用与DNA结合。当μ = 0.1时，二吖啶（1,6-双（9-吖啶基氨基）己烷）表现为单嵌入剂。在这些条件下，两种配体对DNA的分子参数具有同等影响。当μ = 0.001时，二吖啶与DNA的结合模式取决于其在复合物中的浓度。如果一对核苷酸上的二吖啶分子数（r）落在0＜r＜0.2的区域内，其与DNA的结合通过双嵌入模式完成，并伴随着大分子单体残基的结构畸变。当r从0.2增加到0.4时，二吖啶与DNA的结合模式从双嵌入逐渐转变为单嵌入。此外，核苷酸的结构被还原。当μ = 0.001时，单吖啶与DNA复合物的行为与μ = 0.1时观察到的行为相似。
