Silver J, Rabson A, Bryan T, Willey R, Martin M A
Mol Cell Biol. 1987 Apr;7(4):1559-62. doi: 10.1128/mcb.7.4.1559-1562.1987.
Novel endogenous human retroviral sequences were cloned by low-stringency hybridization, using the pol gene of endogenous human retrovirus 51-1. One clone, lambda NP-2, contained gag, pol, env, and long terminal repeat sequences related to the corresponding portions of clone 51-1 and the closely related full-length endogenous human retrovirus 4-1. The sequence of the env gene of NP-2 was 73% homologous to that of 4-1. Genomic Southern blots of male and female DNAs showed that NP-2 is located on the Y chromosome and that the Y chromosome also contains one other sequence closely related to the env and 3' flanking regions of NP-2. Conservation of flanking DNA suggests that the second Y chromosome copy of the NP-2 env sequence arose by gene duplication rather than provirus insertion.
利用内源性人类逆转录病毒51-1的pol基因，通过低严谨度杂交克隆了新型内源性人类逆转录病毒序列。一个克隆体λNP-2包含与克隆体51-1及密切相关的全长内源性人类逆转录病毒4-1的相应部分相关的gag、pol、env和长末端重复序列。NP-2的env基因序列与4-1的env基因序列同源性为73%。男性和女性DNA的基因组Southern杂交显示，NP-2位于Y染色体上，并且Y染色体还包含另一个与NP-2的env及3'侧翼区域密切相关的序列。侧翼DNA的保守性表明，NP-2 env序列在Y染色体上的第二个拷贝是通过基因复制而非前病毒插入产生的。
