O'Brien S J, Bonner T I, Cohen M, O'Connell C, Nash W G
Nature. 1983;303(5912):74-7. doi: 10.1038/303074a0.
The application of recombinant DNA technologies has allowed the detection of at least three families of moderately repetitive DNA segments in the human genome that are homologous to retroviruses previously isolated from mice and primates. One of these DNA segments has been shown by nucleotide sequence comparisons to be distantly related to both Moloney murine leukaemia virus (MoMuLV) and the endogenous baboon retrovirus and to have the sequence organization characteristic of an integrated retrovirus. Isolation of the homologous locus from chimpanzee DNA indicated that the integration event preceded the evolutionary divergence of chimpanzees and man. Here we have used a panel of rodent x human somatic cell hybrids to assign the chromosomal localization of this segment, called ERV1 (endogenous retrovirus-1), to human chromosome 18 (HSA 18).
重组DNA技术的应用使得在人类基因组中检测到至少三类中度重复的DNA片段成为可能，这些片段与先前从小鼠和灵长类动物中分离出的逆转录病毒具有同源性。通过核苷酸序列比较表明，其中一个DNA片段与莫洛尼氏鼠白血病病毒（MoMuLV）和内源性狒狒逆转录病毒都有远缘关系，并且具有整合逆转录病毒的序列组织特征。从黑猩猩DNA中分离出同源基因座表明，整合事件发生在黑猩猩和人类的进化分歧之前。在这里，我们使用一组啮齿动物×人类体细胞杂种，将这个名为ERV1（内源性逆转录病毒-1）的片段定位到人类18号染色体（HSA 18）上。
