College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, P. R. China.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province, Hunan University, Changsha 410082, P. R. China.
Anal Chem. 2022 Sep 6;94(35):12204-12212. doi: 10.1021/acs.analchem.2c02689. Epub 2022 Aug 25.
Theranostics, which combines both diagnostic and therapeutic capabilities in one dose, has always been an intractable challenge in personalized cancer treatment. Herein, a versatile nanotheranostic platform "nanoflare couple (NC)" has been developed for in situ multiplex cancer-related mRNA imaging and subsequent logic-controlled aggregation of gold nanoparticles, leading to gene therapy and photothermal therapy upon irradiation with infrared light. As a proof of concept, TK1 and survivin mRNAs that are highly expressed in most tumor tissues are selected as endogenous cancer indicators and therapy triggers to design the NC. Mice bearing breast cancer cells MCF-7 are prepared as a model to test its efficacy. The in vitro and in vivo assays validate that the NC show the capability for multiplexed mRNA imaging and high efficiency for logic-controlled combinational therapy of breast cancer.
治疗诊断一体化（Theranostics）将诊断和治疗功能集于一体，一直是个性化癌症治疗中的一个棘手挑战。在此，开发了一种多功能的纳米治疗一体化平台“纳米耀斑对（NC）”，用于原位多重癌症相关 mRNA 成像，以及随后在近红外光照射下逻辑控制金纳米颗粒聚集，从而实现基因治疗和光热治疗。作为概念验证，选择在大多数肿瘤组织中高表达的 TK1 和 survivin mRNA 作为内源性癌症标志物和治疗触发物来设计 NC。制备携带乳腺癌细胞 MCF-7 的小鼠作为模型来测试其疗效。体外和体内实验验证了 NC 具有多重 mRNA 成像能力，并且能够高效地进行逻辑控制的乳腺癌组合治疗。
