Department of Obstetrics and Gynecology, Pusan National University School of Medicine, Republic of Korea; Biomedical Research Institute Pusan National University Hospital, Republic of Korea.
The Korea Institute for Public Sperm Bank, Republic of Korea.
Pregnancy Hypertens. 2022 Dec;30:59-67. doi: 10.1016/j.preghy.2022.08.006. Epub 2022 Aug 9.
To assess the association between altered expression of caveolin-1 and p53/p21, as indicatives of cellular senescence, in preeclamptic placenta.
Placental tissues and serum were collected from rats (Sham and reduced uterine perfusion pressure group) at 18.5 days post coitum and humans (normotensive pregnant and preeclampsia groups). The concentration and expression of caveolin-1 were measured in the collected tissues, and the correlation between p53 and p21 expression was evaluation.
Placental mRNA expression and serum concentration of caveolin-1 were measured using qRT-PCR and ELISA, respectively. Altered expressions of caveolin-1 and p53/p21 were revealed and quantified by immunohistochemistry. The association between these changes was investigated using correlation analysis.
Placental mRNA expressions and serum concentrations of caveolin-1 were significantly decreased in reduced uterine perfusion pressure and preeclampsia groups. The expressions of caveolin-1 and p53/ p21 were significantly altered in placenta complicated with preeclampsia. Correlation analysis revealed a significant inverse association between changes in caveolin-1 and p53/p21. Subsequently, these results were obtained by investigating the preeclampsia onset time.
These results revealed that the expression of caveolin-1 profoundly decreases in the placenta and serum of preeclampsia. These factors contribute to the mechanism of accelerated cellular senescence in placenta, which is one of the various etiologies of preeclampsia.
评估小窝蛋白-1 和 p53/p21 的表达改变（作为细胞衰老的指示物）与子痫前期胎盘之间的关系。
在妊娠 18.5 天时，从大鼠（假手术和低子宫灌注压组）和人类（正常妊娠和子痫前期组）收集胎盘组织和血清。测量收集组织中小窝蛋白-1 的浓度和表达，并评估 p53 和 p21 表达之间的相关性。
使用 qRT-PCR 和 ELISA 分别测量胎盘 mRNA 表达和血清中小窝蛋白-1 的浓度。通过免疫组织化学揭示和量化小窝蛋白-1 和 p53/p21 的改变表达。使用相关分析研究这些变化之间的关联。
低子宫灌注压和子痫前期组胎盘 mRNA 表达和血清中小窝蛋白-1 浓度均显著降低。与子痫前期相关的胎盘改变了小窝蛋白-1 和 p53/p21 的表达。相关分析显示小窝蛋白-1 和 p53/p21 之间存在显著的负相关。随后，通过研究子痫前期发病时间获得了这些结果。
这些结果表明，小窝蛋白-1 在胎盘和子痫前期血清中的表达显著降低。这些因素导致胎盘细胞衰老加速的机制，这是子痫前期的多种病因之一。
