Department of Interfacial Phenomena, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Skłodowska University, M. Curie-Skłodowska 3, Lublin 20-031, Poland.
Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, Kraków 30-387, Poland.
Chem Phys Lipids. 2022 Oct;248:105236. doi: 10.1016/j.chemphyslip.2022.105236. Epub 2022 Aug 23.
In order to provide the fundamental information about the interactions of common anionic surfactants with the basic unsaturated phospholipids the influence of three cationic (dodecyltrimethylammonium bromide, DTAB; tetradecyltrimethylammonium bromide, TTAB and hexadecyltrimethylamonium bromide, CTAB) and one anionic (sodium dodecylsulfate, SDS) surfactants on the properties of the 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC) and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) layers was investigated. The studies proved that a tiny amount of the ionic surfactant added to the already synthesized liposome suspension is sufficient to change the zeta potential of the POPC and DOPC liposomes significantly. This impact increases with the surfactant concentration, the alkyl chain length of the surfactant and the degree of lipid saturation. Moreover, this effect is greater for the anionic surfactant than for the cationic one of the same alkyl chain length. The observed findings were confirmed in the course of the research carried out with the use of the corresponding Langmuir monolayers where the surface pressure - mean area isotherms, the compressibility modulus - surface pressure dependences, the monolayer penetration tests, the surface potential - mean molecular area isotherms and Brewster angle microscopy were discussed. It was found that the presence of the surfactants shifts the isotherms towards larger molecular area, to the higher extent for the SDS than DTAB. This effect increases with the increasing surfactant concentration in the subphase. Moreover, the investigated surfactants remain in the monolayer even at high surface pressure. Nevertheless, no effect on the morphology of the POPC and DOPC monolayers was detected from the BAM images. The surface potential and surface charge of the liposomes calculated on the basis of the zeta potential results reflected the interactions between the surfactant and the lipid layers.
为了提供关于常见阴离子表面活性剂与基本不饱和磷脂相互作用的基本信息，研究了三种阳离子（十二烷基三甲基溴化铵、DTAB；十四烷基三甲基溴化铵、TTAB 和十六烷基三甲基溴化铵、CTAB）和一种阴离子（十二烷基硫酸钠、SDS）表面活性剂对 1-棕榈酰基-2-油酰基-甘油-3-磷酸胆碱（POPC）和 1,2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）层性质的影响。研究证明，向已经合成的脂质体悬浮液中添加少量离子表面活性剂足以显著改变 POPC 和 DOPC 脂质体的 zeta 电位。这种影响随着表面活性剂浓度、表面活性剂的烷基链长度和脂质的饱和度而增加。此外，这种效应对于相同烷基链长的阳离子表面活性剂来说比阴离子表面活性剂更大。在使用相应的 Langmuir 单层进行的研究中证实了观察到的发现，讨论了表面压-平均面积等温线、压缩模量-表面压关系、单层穿透试验、表面电位-平均分子面积等温线和布鲁斯特角显微镜。结果发现，表面活性剂的存在将等温线推向更大的分子面积，SDS 比 DTAB 的程度更大。这种效应随着亚相中表面活性剂浓度的增加而增加。此外，即使在高表面压力下，所研究的表面活性剂仍留在单层中。然而，从 BAM 图像中未检测到对 POPC 和 DOPC 单层形态的影响。基于 zeta 电位结果计算的脂质体表面电位和表面电荷反映了表面活性剂与脂质层之间的相互作用。
