Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, MA, USA.
Methods Mol Biol. 2022;2542:271-285. doi: 10.1007/978-1-0716-2549-1_20.
The role of fungal colonizers of the gastrointestinal tract during disease states is not well understood. Antibiotic treatment renders patients highly susceptible to infection by the bacterial pathogen C. difficile while also leading to blooms in fungal commensals, setting the stage for trans-kingdom interactions. Here, we describe a murine model of Candida gastrointestinal colonization coupled to a C. difficile infection (CDI) model, the measurement of CFU of both organisms, and collection of cecum and colon contents for the purpose of quantifying C. difficile toxin production. Additionally, we describe how to induce and purify C. difficile spores.
肠道真菌定植体在疾病状态下的作用尚不清楚。抗生素治疗使患者极易感染细菌病原体艰难梭菌，同时也导致真菌共生体大量繁殖，为跨领域相互作用创造了条件。在这里，我们描述了一种与艰难梭菌感染（CDI）模型相结合的肠道定植的白色念珠菌的小鼠模型，用于测量两种生物体的 CFU，并收集盲肠和结肠内容物以定量艰难梭菌毒素的产生。此外，我们还描述了如何诱导和纯化艰难梭菌孢子。
