Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, China/Guangxi Research Center for the Development of Prepared Chinese Medicines and Ethnic Medicines, Nanning, Guangxi, China.
Guangxi University of Traditional Chinese Medicine, Nanning, Guangxi, China.
Pak J Pharm Sci. 2022 Jul;35(4):1055-1066.
The present study aimed to evaluate the spectrum-effect relationships between high-performance liquid chromatography fingerprints and anti-inflammatory effects of Tetrastigma planicaule(Hook.)Gagnep. Chemical fingerprints of ten batches of Tetrastigma planicaule from various sources were obtained by HPLC. The anti-inflammatory activity was investigated by a model of ear swelling in mice caused by xylene and a model of cotton pellet granuloma. Hierarchical cluster analysis (HCA) results showed that all the samples were clustered into four categories, which was basically consistent with the principal component analysis (PCA) results. The results of the joint grey relational analysis (GRA) and partial least squares regression analysis (PLSR) showed that peaks 1, 2 and 12 were positively correlated with the anti-acute inflammatory effect (ear swelling) in mice, and peaks 3, 5, 6 and 11 were positively correlated with the anti-chronic inflammatory effect (cotton pellet granuloma) in mice. The anti-inflammatory effect of Tetrastigma planicaule is the result of the synergistic effect of multiple components, which provides a basis for further exploring the anti-inflammatory substances and quality evaluation of the herb.
本研究旨在评价高效液相色谱指纹图谱与 Tetrastigma planicaule（Hook.）Gagnep 抗炎作用的谱效关系。采用 HPLC 法得到了来自不同来源的 10 批 Tetrastigma planicaule 的化学指纹图谱。通过二甲苯致小鼠耳肿胀模型和棉粒肉芽肿模型考察其抗炎活性。层次聚类分析（HCA）结果表明，所有样品基本分为 4 类，与主成分分析（PCA）结果基本一致。联合灰色关联分析（GRA）和偏最小二乘回归分析（PLSR）结果表明，峰 1、2 和 12 与小鼠的急性抗炎作用（耳肿胀）呈正相关，峰 3、5、6 和 11 与小鼠的慢性抗炎作用（棉粒肉芽肿）呈正相关。 Tetrastigma planicaule 的抗炎作用是多种成分协同作用的结果，为进一步探讨其抗炎物质和质量评价提供了依据。
