未经处理和处理后的污水中的 ESKAPEE 病原体的抗药基因组：波兰案例研究。

The Resistome of ESKAPEE Pathogens in Untreated and Treated Wastewater: A Polish Case Study.

机构信息

Department of Water Protection Engineering and Environmental Microbiology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-720 Olsztyn, Poland.

Department of Environmental Biotechnology, Faculty of Geoengineering, University of Warmia and Mazury in Olsztyn, 10-709 Olsztyn, Poland.

出版信息

Biomolecules. 2022 Aug 21;12(8):1160. doi: 10.3390/biom12081160.

DOI:10.3390/biom12081160

PMID:36009054

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9405806/

Abstract

The aim of this study was to quantify ESKAPEE bacteria, genes encoding resistance to antibiotics targeting this group of pathogens, as well as integrase genes in municipal wastewater and river water. Environmental DNA was extracted from the collected samples and used in deep sequencing with the Illumina TruSeq kit. The abundance of bacterial genera and species belonging to the ESKAPEE group, 400 ARGs associated with this microbial group, and three classes of integrase genes were determined. A taxonomic analysis revealed that was the dominant bacterial genus, whereas and were the dominant bacterial species. The analyzed samples were characterized by the highest concentrations of the following ARGs: , , , B, and S. , , and genes encoding resistance to β-lactams (, , , , , and ) and fluoroquinolones (S) were detected in samples of river water collected downstream from the wastewater discharge point. The correlation analysis revealed a strong relationship between (bacterial species regarded as an emerging human pathogen) and genes encoding resistance to all tested groups of antimicrobials. The transmission of the studied bacteria (in particular ) and ARGs to the aquatic environment poses a public health risk.

摘要

本研究旨在量化市政污水和河水环境中的 ESKAPEE 细菌、针对该组病原体的抗生素耐药基因以及整合酶基因。从采集的样本中提取环境 DNA，并使用 Illumina TruSeq 试剂盒进行深度测序。确定了属于 ESKAPEE 组的细菌属和种的丰度、与该微生物组相关的 400 个 ARG 以及三类整合酶基因。分类分析表明，是优势细菌属，而和是优势细菌种。分析样本的特征是以下 ARG 浓度最高：,,, B 和 S. 、和编码对β-内酰胺类（、、、、和）和氟喹诺酮类（S）的耐药基因在从废水排放点下游采集的河水样本中被检测到。相关性分析表明，（被认为是一种新兴人类病原体的细菌种）与编码对所有测试组抗生素的耐药基因之间存在很强的关系。研究细菌（特别是）和 ARG 向水生环境的传播对公共健康构成威胁。