Yu Xue, Jiang Kan, Zhang Wei, Dong Shuqing, Wu Yujie, Zhang Gaosen, Wu Shiyu, Chen Tuo, Liu Guangxiu
Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730030, China.
Key Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730030, China.
Antioxidants (Basel). 2022 Jul 29;11(8):1493. doi: 10.3390/antiox11081493.
The genus is a source of many natural products that are critical in the development of new medicines. Here, we isolated a novel carotenoid from sp. QL17 and characterized its properties. The carotenoid was extracted with methanol, and purified by column chromatography and semi-preparative HPLC. Based on micrOTOF-Q and NMR analyses, the pigment was chemically characterized as 2,2'-((((1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,14,18-tetramethylicosa-1,3,5,7,9,11,13,15,17,19-decaene-1,20-diyl)bis(2,2,4-trimethylcyclohex-3-ene-3,1-diyl)) bis(ethan-2-yl-1-ylidene))bi(propane-1,3-diol), and named arthroxanthin. The biological activities of arthroxanthin were evaluated with DPPH, ABTS and MTT assays. Arthroxanthin exhibited excellent radical scavenging properties, as shown for 2, 20-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-n-(3,2-ethyl-benzothiazole-6-sulfonic acid) ammonium salt (ABTS), respectively, with IC50s of 69.8 and 21.5 µg/mL. It also showed moderate anticancer activities against HepG2, Hela, MDAB-231, SW480, and MKN-45 with IC50 values of 107.6, 150.4, 143.4, 195.9, and 145.5 μg/mL, respectively. Therefore, arthroxanthin derived from sp. QL17 may be a potent antioxidant and anticancer agent for food and pharmaceutical use.
该属是许多天然产物的来源，这些天然产物对新药的开发至关重要。在此，我们从QL17菌株中分离出一种新型类胡萝卜素并对其性质进行了表征。该类胡萝卜素用甲醇提取，通过柱色谱和半制备型高效液相色谱进行纯化。基于微量飞行时间质谱仪（micrOTOF-Q）和核磁共振（NMR）分析，该色素经化学鉴定为2,2'-((((1E,3E,5E,7E,9E,11E,13E,15E,17E,19E)-3,7,14,18-四甲基二十碳-1,3,5,7,9,11,13,15,17,19-癸烯-基-1,20-二基)双(2,2,4-三甲基环己-3-烯-3,1-二基))双(乙烷-2-亚基-1-亚基))双(丙烷-1,3-二醇)，并命名为节旋藻黄素。用DPPH、ABTS和MTT试验评估了节旋藻黄素的生物活性。节旋藻黄素表现出优异的自由基清除性能，分别对2,2-二苯基-1-苦基肼（DPPH）和2,2'-氮-(3-乙基苯并噻唑啉-6-磺酸)二铵盐（ABTS）而言，其半数抑制浓度（IC50）分别为69.8和21.5μg/mL。它对肝癌细胞HepG2、宫颈癌HeLa细胞、乳腺癌MDAB-231细胞、结肠癌细胞SW480和胃癌细胞MKN-45也表现出中等的抗癌活性，IC50值分别为107.6、150.4、143.4、195.9和145.5μg/mL。因此，源自QL17菌株的节旋藻黄素可能是一种用于食品和制药的强效抗氧化剂和抗癌剂。
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