University of Bonn, Institute of Nutritional and Food Science, Food Microbiology and Hygiene, Endenicher Allee 19B, 53115 Bonn, Germany.
University of Bonn, Institute of Nutritional and Food Science, Molecular Food Technology, Endenicher Allee 19B, 53115 Bonn, Germany.
Int J Syst Evol Microbiol. 2020 May;70(5):3027-3036. doi: 10.1099/ijsem.0.004125.
A pink-coloured bacterium (strain KR32) was isolated from cheese and assigned to the ' group'. Members of the 'pink group' form a stable clade (100 % bootstrap value) and contain the species , and , which share ≥99.0 % 16S rRNA gene sequence similarity. Isolate KR32 showed highest 16S rRNA gene sequence similarity (99.9 %) to DSM 20550. Additional multilocus sequence comparison confirmed the assignment of strain KR32 to the clade 'pink group'. Average nucleotide identity and digital DNA-DNA hybridization values between isolate KR32 and DSM 20550 were 82.85 and 26.30 %, respectively. The G+C content of the genomic DNA of isolate KR32 was 69.14 mol%. Chemotaxonomic analysis determined anteiso-C as the predominant fatty acid and MK-9(H) as the predominant menaquinone. Polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and monoacyldimannosyl-monoacylglycerol. The peptidoglycan type of the isolate was A3α. The carotenoid bacterioruberin was detected as the major pigment. At 10 °C, strain KR32 grew with increased concentrations of bacterioruberin and production of unsaturated fatty acids. Strain KR32 was a Gram-stain-positive, catalase-positive, oxidase-positive and coccus-shaped bacterium with optimal growth at 27-30 °C and pH 8. The results of phylogenetic and phenotypic analyses enabled the differentiation of the isolate from other closely related species of the 'pink group'. Therefore, strain KR32 represents a novel species for which the name sp. nov. is proposed. The type strain is KR32 (=DSM 109896=LMG 31480=NCCB 100733).
从奶酪中分离到一株粉红色细菌（菌株 KR32），并将其归入“粉红群”。“粉红群”的成员形成一个稳定的分支（100%的自举值），包含种、和，它们的 16S rRNA 基因序列相似性≥99.0%。菌株 KR32 与 DSM 20550 的 16S rRNA 基因序列相似性最高（99.9%）。附加的多位点序列比较确认了菌株 KR32 属于“粉红群”分支。菌株 KR32 与 DSM 20550 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 82.85%和 26.30%。菌株 KR32 的基因组 DNA G+C 含量为 69.14mol%。化学分类分析确定anteiso-C 为主要脂肪酸，MK-9(H) 为主要menaquinone。极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰肌醇和单酰基二甘露糖单酰基甘油。该分离株的肽聚糖类型为 A3α。主要色素为细菌叶红质。在 10°C 下，菌株 KR32 随着细菌叶红质浓度的增加和不饱和脂肪酸的产生而生长。菌株 KR32 是革兰氏阳性、过氧化氢酶阳性、氧化酶阳性的球菌，最佳生长温度为 27-30°C，pH 值为 8。系统发育和表型分析的结果使该分离株与“粉红群”中其他密切相关的种区分开来。因此，菌株 KR32 代表一个新种，建议命名为 sp. nov.。模式株为 KR32（=DSM 109896=LMG 31480=NCCB 100733）。
