Exploration of Molecular Structure, DFT Calculations, and Antioxidant Activity of a Hydrazone Derivative.

The hydrazine derivatives are known to possess several biological activities including anticancer, antibacterial and anti-fungal, anticonvulsant, and antioxidant. This communication presents the synthesis, X-ray crystal structure analysis, DFT calculations, cell cytotoxicity, and antioxidant activity of the Schiff base 4,4'-((1E,1'E)-hydrazine-1,2-diylidenebis(ethan-1-yl-1-ylidene))bis(benzene-1,3-diol) (compound ). We have also isolated the side product compound and characterized it using single X-ray crystallography. The crystal structure of compound depicts that the ensuing C-H···N hydrogen bonding interaction is presented and discussed herein. In addition, the calculations using density functional theory (DFT) approximation supported by experimental H and C NMR studies on the key compound are reported. The results of theoretical and experimental H and C NMR were concordant. The antioxidant activity of compound was determined by using 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation assays and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical assay. Compound demonstrated excellent antioxidant activity in ABTS assay (IC = 4.30 ± 0.21 µM) and DPPH assay (IC = 81.06 ± 0.72 µM) with almost no cytotoxicity below 25 µM.