Catarino Marcelo D, Circuncisão Ana Rita, Neves Bruno, Marçal Catarina, Silva Artur M S, Cruz Maria Teresa, Cardoso Susana M
LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal.
Department of Medical Sciences and Institute of Biomedicine-iBiMED, University of Aveiro, 3810-193 Aveiro, Portugal.
Antioxidants (Basel). 2022 Aug 4;11(8):1518. doi: 10.3390/antiox11081518.
A phlorotannin extract was obtained from , revealing a profile rich in fucophlorethol-type and carmalol-type compounds. When subjected to simulated gastrointestinal digestion, its levels of total phlorotannins and antioxidant activity, measured in vitro via NO and O scavenging assays, were reduced, thus suggesting that these compounds' integrity and bioactivity are negatively affected by the digestive process. Nevertheless, when undigested vs. digested extracts were used on lipopolysaccharide-stimulated Raw 264.7 macrophages, both showed a strong inhibitory effect on the cellular NO production. In fact, although not statistically significant, the digested extract revealed a tendentially stronger effect compared to its undigested counterpart, suggesting that even though there is a decrease in the phlorotannins' concentration after digestion, with a consequent loss of their scavenging properties, the possible degradation products being formed may exert their effects through the modulation of the intracellular signaling mechanisms. Overall, this study not only contributes to a better understanding of the phlorotannins' composition of the species , but also shows that, although the digestive process may affect the integrity and concentration of these compounds, this does not necessarily translate into loss of bioactivity, in particular the anti-inflammatory activity, probably owing to the bioactive effects that the degradation products of these phenolics may have at an intracellular level.
从……中获得了一种间苯三酚单宁提取物，结果显示其富含岩藻黄醇型和卡马醇型化合物。在模拟胃肠道消化过程中，通过一氧化氮和超氧阴离子清除试验在体外测量其总间苯三酚单宁水平和抗氧化活性有所降低，这表明这些化合物的完整性和生物活性受到消化过程的负面影响。然而，当将未消化提取物与消化后的提取物用于脂多糖刺激的Raw 264.7巨噬细胞时，两者均对细胞一氧化氮的产生显示出强烈的抑制作用。实际上，尽管没有统计学意义，但消化后的提取物与其未消化的对应物相比显示出更强的趋势性作用，这表明尽管消化后间苯三酚单宁的浓度降低，其清除特性随之丧失，但可能形成的降解产物可能通过调节细胞内信号传导机制发挥作用。总体而言，本研究不仅有助于更好地了解该物种间苯三酚单宁的组成，还表明尽管消化过程可能会影响这些化合物的完整性和浓度，但这不一定会转化为生物活性的丧失，特别是抗炎活性，这可能是由于这些酚类化合物的降解产物在细胞内水平可能具有的生物活性作用。
