Bai Kaiwen, Jiang Luyi, Li Qiming, Zhang Jingfei, Zhang Lili, Wang Tian
School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China.
College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Antioxidants (Basel). 2022 Aug 10;11(8):1550. doi: 10.3390/antiox11081550.
The current study sought to understand the mechanism underlying skeletal muscle dysfunction brought on by intrauterine growth restriction (IUGR) and to explore the treatment benefits of applying dimethylglycine sodium salt (DMG-Na) in sow milk to newborns during the suckling period. Each of the 10 sows delivered one newborn with a normal birth weight (NBW) and one with an IUGR. Additionally, two NBW and two IUGR newborns were collected per litter of another 10 sows. The 20 NBW newborns were divided between the N (sow milk) and ND (sow milk + 0.1% DMG-Na) groups, while 20 IUGR newborns were divided between the I (sow milk) and ID (sow milk + 0.1% DMG-Na) groups. The skeletal muscle histomorphology, redox status, and levels of gene and protein expression were worse (p < 0.05) in the I group than in the N group. In addition, supplementation with DMG-Na (ND and ID groups) improved (p < 0.05) those parameters compared to the unsupplemented groups (N and I groups). Inhibited nuclear factor erythroid 2-related factor 2 (Nrf2)/sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptorγcoactivator-1α (PGC-1α) activity resulted in decreased redox status, skeletal muscle structural damage, skeletal muscle mitochondrial function impairment, and decreased performance in IUGR newborns. Supplementation of DMG-Na in sow milk activated the Nrf2/SIRT1/PGC-1α in IUGR newborns, thereby improving their skeletal muscle performance.
当前的研究旨在了解宫内生长受限（IUGR）导致骨骼肌功能障碍的潜在机制，并探索在哺乳期给新生仔猪投喂添加二甲基甘氨酸钠盐（DMG-Na）的母猪乳汁的治疗效果。10头母猪每头分娩1头出生体重正常（NBW）的新生仔猪和1头IUGR新生仔猪。此外，从另外10头母猪的每窝仔猪中收集2头NBW新生仔猪和2头IUGR新生仔猪。20头NBW新生仔猪被分为N组（母猪乳汁）和ND组（母猪乳汁 + 0.1% DMG-Na），而20头IUGR新生仔猪被分为I组（母猪乳汁）和ID组（母猪乳汁 + 0.1% DMG-Na）。I组的骨骼肌组织形态学、氧化还原状态以及基因和蛋白质表达水平均比N组差（p < 0.05）。此外，与未添加组（N组和I组）相比，添加DMG-Na（ND组和ID组）改善了（p < 0.05）这些参数。核因子红细胞2相关因子2（Nrf2）/沉默调节蛋白1（SIRT1）/过氧化物酶体增殖物激活受体γ共激活因子-1α（PGC-1α）活性受到抑制，导致IUGR新生仔猪的氧化还原状态降低、骨骼肌结构损伤、骨骼肌线粒体功能受损以及性能下降。在母猪乳汁中添加DMG-Na可激活IUGR新生仔猪的Nrf2/SIRT1/PGC-1α，从而改善其骨骼肌性能。
