Delsante Costanza, Pinna Carlo, Sportelli Federica, Dalmonte Thomas, Stefanelli Claudio, Vecchiato Carla G, Biagi Giacomo
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Department for Life Quality Studies, University of Bologna, Corso d'Augusto 237, 47921 Rimini, Italy.
Animals (Basel). 2022 Aug 17;12(16):2100. doi: 10.3390/ani12162100.
Microalgae are a source of bioactive compounds having recently been studied for their possible application as health-promoting ingredients. The aim of the study was to evaluate in an in vitro canine gut model the effects of four microalgae, Arthrospira platensis (AP), Haematococcus pluvialis (HP), Phaeodactylum tricornutum (PT) and Chlorella vulgaris (CV), on some fecal microbial populations and metabolites. The four microalgae were subjected to an in vitro digestion procedure, and subsequently, the digested biomass underwent colonic in vitro fermentation. After 6 h of incubation, PT increased propionate (+36%) and butyrate (+24%), and decreased total BCFA (-47%), isobutyrate (-52%) and isovalerate (-43%) and C. hiranonis (-0.46 log10 copies/75 ng DNA). After 24 h, PT increased propionate (+21%) and isovalerate (+10%), and decreased the abundance of Turicibacter spp. (7.18 vs. 6.69 and 6.56 log10 copies/75 ng DNA for CTRL vs. PT, respectively); moreover, after 24 h, CV decreased C. coccoides (-1.12 log10 copies/75 ng DNA) and Enterococcus spp. (-0.37 log10 copies/75 ng DNA). In conclusion, the microbial saccharolytic activities and the shift in fecal bacterial composition were less pronounced than expected, based on current literature. This study should be considered as a preliminary assessment, and future investigations are required to better understand the role of microalgae in canine nutrition.
微藻是生物活性化合物的来源，最近人们对其作为促进健康成分的可能应用进行了研究。本研究的目的是在体外犬肠道模型中评估四种微藻，即钝顶螺旋藻（AP）、雨生红球藻（HP）、三角褐指藻（PT）和普通小球藻（CV），对一些粪便微生物种群和代谢产物的影响。对这四种微藻进行体外消化程序，随后，消化后的生物质进行结肠体外发酵。孵育6小时后，PT增加了丙酸（+36%）和丁酸（+24%），并降低了总支链脂肪酸（-47%）、异丁酸（-52%）和异戊酸（-43%）以及平成希拉肠杆菌（-0.46 log10拷贝/75 ng DNA）。24小时后，PT增加了丙酸（+21%）和异戊酸（+10%），并降低了Turicibacter spp.的丰度（分别为对照与PT相比，7.18对6.69和6.56 log10拷贝/75 ng DNA）；此外，24小时后，CV降低了球状梭菌（-1.12 log10拷贝/75 ng DNA）和肠球菌属（-0.37 log10拷贝/75 ng DNA）。总之，基于当前文献，微生物的糖分解活性和粪便细菌组成的变化不如预期明显。本研究应被视为初步评估，需要进一步的研究来更好地理解微藻在犬类营养中的作用。
