Yar Asfand, Choudary Muhammad Adil, Rehman Abdul, Hussain Abid, Elahi Amina, Ur Rehman Farooq, Waqar Ahmed Bilal, Alshammari Abdulrahman, Alharbi Metab, Nisar Muhammad Atif, Khurshid Mohsin, Khan Zaman
University Institute of Medical Laboratory Technology, Faculty of Allied Health Sciences, The University of Lahore, Lahore 54000, Pakistan.
Central Park Medical College, Lahore 54000, Pakistan.
Antibiotics (Basel). 2022 Aug 4;11(8):1061. doi: 10.3390/antibiotics11081061.
Rapid urbanization has increased human-animal interaction and consequently enhanced the chances to acquire zoonotic diseases. The current investigation is focused to uncover the genetic diversity of multidrug-resistant strains between different ecologies (i.e., humans, livestock, and environment) at the molecular level by employing antimicrobial resistance profiling, virulence genes profiling, and microbial typing approach using ERIC PCR. Based on multiple antibiotic resistance, overall, 19 antibiotic resistance patterns (R1-R19) were observed. Most of the strains (49/60) were detected to have the combinations of , , and genes and considered STEC/EPEC/EHEC. A total of 18 unique genetic profiles were identified based on ERIC-PCR fingerprints and most of the strains (13) belong to P1 whereas the least number of strains were showing profiles P7 and P8-P11 (one member each profile). The calculated values for Shannon index (H) for human, animal, and environment are 1.70, 1.82, and 1.78, respectively revealing the highest genetic diversity among the strains of animal origin. The study revealed that drug-resistant pathogenic strains could be transmitted bidirectionally among the environment, humans, and animals.
快速城市化增加了人与动物的互动，从而增加了感染人畜共患病的机会。当前的调查旨在通过采用抗菌药物耐药性分析、毒力基因分析以及使用ERIC PCR的微生物分型方法，在分子水平上揭示不同生态环境（即人类、牲畜和环境）中多重耐药菌株的遗传多样性。基于多重抗生素耐药性，总体上观察到19种抗生素耐药模式（R1-R19）。大多数菌株（49/60）被检测到具有、和基因的组合，并被认为是STEC/EPEC/EHEC。基于ERIC-PCR指纹图谱共鉴定出18种独特的遗传图谱，大多数菌株（13株）属于P1，而显示P7和P8-P11图谱的菌株数量最少（每个图谱各1株）。人类、动物和环境的香农指数（H）计算值分别为1.70、1.82和1.78，这表明动物源菌株的遗传多样性最高。该研究表明，耐药病原菌可在环境、人类和动物之间双向传播。
