Tomlinson A, Durbin J, Coupland R E
Neuroscience. 1987 Mar;20(3):895-904. doi: 10.1016/0306-4522(87)90250-8.
A morphometric analysis of normal Wistar rat adrenal medulla following perfusion fixation and Araldite embedding, was correlated with catecholamine levels on fresh tissue, measured by high-performance liquid chromatography. The mean volume of whole adrenal is 13.2 mm3 and the mean medullary volume 1.3 mm3. Volume density estimates showed that the medulla is composed of 63% chromaffin tissue with an adrenaline to noradrenaline storing cell ratio of 4.4:1. The vasculature occupies 20%, neuronal tissue 5% and interstitial tissues 12% of the medulla. A comparison was made of cell volumes, cell numbers and volume and surface density estimates of cytoplasmic organelles in adrenaline and noradrenaline storing cells. The mean cell volume of adrenaline storing cells at 1300 micron3 is larger than that of noradrenaline storing cells at 980 micron3. A single adrenal medulla contains 4.4-5.7 X 10(5) adrenaline cells and 1.5-1.9 X 10(5) noradrenaline cells. Chromaffin granules account for approximately 30% of the volume of the cytoplasm; the numerical density of granules at different sites in the cell was calculated for adrenaline cells. The volume density of mitochondria (4%) and the surface density of mitochondrial membranes (the ratio of outer to inner membrane being approximately 1:2.3) were similar in both cell types. Rough endoplasmic reticulum was the only organelle to show a significant difference in volume and surface density between the two cell types. Adrenaline storing cells have stacks of rough endoplasmic reticulum which have two to three times the surface and volume densities of that found diffusely scattered throughout noradrenaline cells. The adrenaline content of an adrenaline storing cell is 0.14 X 10(-6) microM and that of a granule 3.0 X 10(-12) or 3.8 X 10(-12) mumoles depending on the method of calculation. The noradrenaline content of noradrenaline storing cells can only be calculated on the assumption that all noradrenaline is stored in this cell type though it is likely that some is contained within adrenaline cells. Based on this assumption the noradrenaline content is 0.17 X 10(-6) mumoles per cell and 5 X 10(-12) mumoles per granule. The present study provides baseline morphometric data on the rat adrenal medulla at tissue and cellular level correlated with amine levels in adrenaline and noradrenaline storing cells and granules.
对经灌注固定和环氧树脂包埋的正常Wistar大鼠肾上腺髓质进行形态计量分析，并与通过高效液相色谱法测定的新鲜组织中的儿茶酚胺水平相关联。整个肾上腺的平均体积为13.2立方毫米，髓质平均体积为1.3立方毫米。体积密度估计显示，髓质由63%的嗜铬组织组成，肾上腺素储存细胞与去甲肾上腺素储存细胞的比例为4.4:1。脉管系统占髓质的20%，神经组织占5%，间质组织占12%。对肾上腺素和去甲肾上腺素储存细胞中的细胞体积、细胞数量以及细胞质细胞器的体积和表面密度估计进行了比较。肾上腺素储存细胞的平均细胞体积为1300立方微米，大于去甲肾上腺素储存细胞的980立方微米。单个肾上腺髓质包含4.4 - 5.7×10⁵个肾上腺素细胞和1.5 - 1.9×10⁵个去甲肾上腺素细胞。嗜铬颗粒约占细胞质体积的30%；计算了肾上腺素细胞中细胞不同部位颗粒的数量密度。两种细胞类型中线粒体的体积密度（4%）和线粒体膜的表面密度（外膜与内膜的比例约为1:2.3）相似。粗面内质网是两种细胞类型中唯一在体积和表面密度上显示出显著差异的细胞器。肾上腺素储存细胞有粗面内质网堆叠，其表面密度和体积密度是在去甲肾上腺素细胞中分散分布的粗面内质网的两到三倍。一个肾上腺素储存细胞的肾上腺素含量为0.14×10⁻⁶微摩尔，根据计算方法不同，一个颗粒的肾上腺素含量为3.0×10⁻¹²或3.8×10⁻¹²微摩尔。去甲肾上腺素储存细胞的去甲肾上腺素含量只能在假设所有去甲肾上腺素都储存在这种细胞类型中的情况下计算，尽管可能有一些包含在肾上腺素细胞中。基于此假设，每个细胞的去甲肾上腺素含量为0.17×10⁻⁶微摩尔每个颗粒为5×10⁻¹²微摩尔。本研究提供了大鼠肾上腺髓质在组织和细胞水平的基线形态计量数据，并与肾上腺素和去甲肾上腺素储存细胞及颗粒中的胺水平相关联。
