Jousselin-Hosaja M
Université Pierre et Marie Curie, CNRS, Departément de Cytologie, Paris, France.
J Endocrinol. 1988 Jan;116(1):149-53. doi: 10.1677/joe.0.1160149.
The effects of long-term transplantation on the ultrastructure of adrenaline- and noradrenaline-storing cells from the adrenal medulla were determined using morphometric methods. Mouse adrenal medulla were freed from the adrenal cortex and grafted into the occipital cortex of the brain. Two types of chromaffin cells were identified by electron microscopy in grafts fixed with glutaraldehyde and osmium tetroxide. Noradrenaline-type cells were predominant and formed 70-80% of the surviving population of grafted chromaffin cells. A minority of the chromaffin cells contained medium-sized granules (140-210 nm in diameter) (medium granule cell; MGC) with finely granular moderately electron dense cores. Morphometric analysis of noradrenaline phenotype cells and MGC cells in transplants showed no significant differences compared with the noradrenaline-storing cells of normal adrenal glands. In contrast, noradrenaline-type cells and MGC cells in the grafts had areas of secretory vesicles which were significantly (P less than 0.01) larger and areas of rough endoplasmic reticulum which were significantly (P less than 0.01) smaller than those of the adrenaline-storing cells of normal adrenal glands. It was concluded that long-term transplantation caused no degenerative changes in the ultrastructure of mouse adrenal chromaffin cells.
采用形态计量学方法确定长期移植对肾上腺髓质中储存肾上腺素和去甲肾上腺素细胞超微结构的影响。将小鼠肾上腺髓质从肾上腺皮质分离出来，移植到脑枕叶皮质。在用戊二醛和四氧化锇固定的移植物中，通过电子显微镜鉴定出两种嗜铬细胞。去甲肾上腺素型细胞占主导地位，占移植嗜铬细胞存活群体的70 - 80%。少数嗜铬细胞含有中等大小的颗粒（直径140 - 210 nm）（中等颗粒细胞；MGC），其核心为细颗粒状、中等电子密度。对移植中去甲肾上腺素表型细胞和MGC细胞的形态计量分析显示，与正常肾上腺储存去甲肾上腺素的细胞相比，无显著差异。相反，移植物中的去甲肾上腺素型细胞和MGC细胞的分泌小泡面积显著（P小于0.01）更大，粗面内质网面积显著（P小于0.01）小于正常肾上腺储存肾上腺素的细胞。得出的结论是，长期移植未引起小鼠肾上腺嗜铬细胞超微结构的退行性变化。
