Dajas F, Bolioli B, Castello M E, Silveira R
Neurosci Lett. 1987 Jun 1;77(1):87-91. doi: 10.1016/0304-3940(87)90612-4.
It has been shown that Fasciculins (FAS), polypeptides isolated from the venom of the green mamba Dendroaspis angusticeps, provoke a powerful inhibition of peripheral acetylcholinesterase (AChE). In the present study, 0.5 microliter of increasing concentrations (10-500 micrograms/ml) of FAS were injected into the striatum of rats. Micropunches taken 2 mm around the injection site showed 90% inhibition of AChE up to 24 h after FAS injection (500 micrograms/ml). AChE activity was about 50% of controls at the 7th day without apparent cell loss. Assessment of AChE activity in the whole striatum showed no inhibition. It is postulated that, due to this potent, localized and long-lasting central nervous system AChE inhibition, FAS could become a useful tool for the study of central cholinergic pathways.
研究表明，从绿曼巴蛇（Dendroaspis angusticeps）毒液中分离出的束丝菌素（FAS）能强烈抑制外周乙酰胆碱酯酶（AChE）。在本研究中，将0.5微升浓度递增（10 - 500微克/毫升）的FAS注入大鼠纹状体。在注射部位周围2毫米处采集的微量样本显示，注射FAS（500微克/毫升）后长达24小时，AChE受到90%的抑制。在第7天，AChE活性约为对照组的50%，且无明显细胞损失。对整个纹状体中AChE活性的评估显示没有抑制作用。据推测，由于这种对中枢神经系统AChE的强效、局部且持久的抑制作用，FAS可能成为研究中枢胆碱能通路的有用工具。
