State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 310029, China.
Int J Mol Sci. 2022 Aug 12;23(16):9015. doi: 10.3390/ijms23169015.
The DExD/H-box protein family encompasses a large number of RNA helicases that are involved in RNA metabolism and a variety of physiological functions in different species. However, there is limited knowledge of whether DExD/H-box proteins play a role in the pathogenicity of plant fungal pathogens. In the present work, the DExD/H-box protein MoDHX35, which belongs to the DEAH subfamily, was shown to be crucial in appressoria formation and full virulence of the rice blast fungus, . The predicted protein sequence of MoDHX35 had typical DEAH-box domains, showed 47% identity to DHX35 in species, but had no orthologs in . Deletion of the gene resulted in reduced tolerance of the mutants to doxorubicin, a nucleic acid synthesis disturbing agent, suggesting the involvement of in RNA metabolism. -deleted mutants exhibited normal vegetative growth, conidia generation and conidial germination, but showed a reduced appressorium formation rate and attenuated virulence. Our work demonstrates the involvement of DEAH-box protein functions in the pathogenicity of plant fungal pathogens.
DExD/H 框蛋白家族包含大量参与 RNA 代谢和不同物种中多种生理功能的 RNA 解旋酶。然而，关于 DExD/H 框蛋白是否在植物真菌病原体的致病性中发挥作用，知之甚少。在本工作中，属于 DEAH 亚家族的 DExD/H 框蛋白 MoDHX35 被证明在稻瘟病菌的附着胞形成和完全毒力中至关重要。MoDHX35 的预测蛋白序列具有典型的 DEAH 盒结构域，与 物种中的 DHX35 具有 47%的同一性，但在 中没有同源物。该基因的缺失导致突变体对多柔比星（一种核酸合成干扰剂）的耐受性降低，表明其参与 RNA 代谢。-缺失突变体表现出正常的营养生长、分生孢子生成和分生孢子萌发，但附着胞形成率降低，毒力减弱。我们的工作表明 DEAH 框蛋白功能参与了植物真菌病原体的致病性。
