Stress Imunnity Pathogens (SIMPA), Université de Lorraine, F-54000 Nancy, France.
CHRU de Nancy, Service de Microbiologie, F-54000 Nancy, France.
Int J Mol Sci. 2022 Aug 20;23(16):9411. doi: 10.3390/ijms23169411.
The health-promoting , which is part of the core microbiome, has recently received a lot of attention, showing beneficial properties for its host and potential as a new biotherapeutic product. However, no study has yet investigated the cell surface molecules and structures of that allow its maintenance within the gut microbiota. Moreover, although is strongly recognized as an intestinal commensal species with benefits for its host, several works displayed controversial results, showing it as an opportunistic pathogen. In this study, we reported gene clusters potentially involved in the synthesis of capsule, fimbriae-like and pili-like cell surface structures in 26 genomes and applied the new RfbA-typing classification in order to better understand and characterize the beneficial/pathogenic behavior related to strains. Two different types of fimbriae, three different types of pilus and up to fourteen capsular polysaccharide loci were identified over the 26 genomes studied. Moreover, the addition of data to the -type classification modified the outcome by rearranging genes and adding a fifth group to the classification. In conclusion, the strain variability in terms of external proteinaceous structure could explain the inter-strain differences previously observed of adhesion capacities and its potential pathogenicity, but no specific structure related to beneficial or detrimental activity was identified.
具有促进健康作用的菌，是核心微生物群的一部分，最近受到了广泛关注，其对宿主具有有益特性，并有可能成为一种新的生物治疗产品。然而，目前还没有研究调查允许其在肠道微生物群中维持的菌的细胞表面分子和结构。此外，尽管被强烈认为是一种对宿主有益的肠道共生种，但有几项研究显示出有争议的结果，表明它是一种机会性病原体。在本研究中，我们报告了在 26 个基因组中可能参与荚膜、菌毛样和类菌毛样细胞表面结构合成的基因簇，并应用了新的 RfbA 分型分类方法，以便更好地理解和表征与菌株相关的有益/致病行为。在研究的 26 个基因组中，鉴定出了两种不同类型的菌毛、三种不同类型的纤毛和多达 14 个荚膜多糖基因座。此外，向 - 型分类中添加数据通过重新排列基因并将分类添加到第五组来改变结果。总之，在菌的外蛋白结构方面的菌株可变性可以解释先前观察到的菌粘附能力及其潜在致病性的菌株间差异，但没有鉴定出与菌有益或有害活性相关的特定结构。
