College of Pharmacy, Yonsei Institute of Pharmaceutical Sciences, Yonsei University, Incheon 21983, Korea.
Department of Global Innovative Drug, The Graduate School of Chung-Ang University, Seoul 06974, Korea.
Int J Mol Sci. 2022 Aug 22;23(16):9467. doi: 10.3390/ijms23169467.
The fibroblast growth factor (FGF) family has various biological functions, including cell growth, tissue regeneration, embryonic development, metabolism, and angiogenesis. In the case of hair growth, several members of the FGF family, such as FGF1 and FGF2, are involved in hair growth, while FGF5 has the opposite effect. In this study, the regulation of the hair growth cycle by FGF12 was investigated. To observe its effect, the expression of FGF12 was downregulated in mice and outer root sheath (ORS) by siRNA transfection, while FGF12 overexpression was carried out using FGF12 adenovirus. For the results, FGF12 was primarily expressed in ORS cells with a high expression during the anagen phase of hair follicles. Knockdown of FGF12 delayed telogen-to-anagen transition in mice and decreased the hair length in vibrissae hair follicles. It also inhibited the proliferation and migration of ORS cells. On the contrary, FGF12 overexpression increased the migration of ORS cells. FGF12-overexpressed ORS cells induced the telogen-to-anagen transition in the animal model. In addition, FGF12 overexpression regulated the expression of PDGF-CC, MDK, and HB-EGF, and treatment of these factors exhibited hair growth promotion. Altogether, FGF12 promoted hair growth by inducing the anagen phase of hair follicles, suggesting the potential for hair loss therapy.
成纤维细胞生长因子（FGF）家族具有多种生物学功能，包括细胞生长、组织再生、胚胎发育、代谢和血管生成。在毛发生长方面，FGF 家族的几个成员，如 FGF1 和 FGF2，参与毛发生长，而 FGF5 则有相反的作用。在这项研究中，研究了 FGF12 对毛发生长周期的调节作用。为了观察其效果，通过 siRNA 转染下调了小鼠和外根鞘（ORS）中的 FGF12 表达，同时使用 FGF12 腺病毒过表达 FGF12。结果表明，FGF12 主要在外根鞘细胞中表达，在毛囊的生长期表达水平较高。FGF12 的敲低延迟了小鼠的休止期向生长期的转变，并减少了触须毛囊的毛发生长长度。它还抑制了 ORS 细胞的增殖和迁移。相反，FGF12 的过表达增加了 ORS 细胞的迁移。FGF12 过表达的 ORS 细胞在动物模型中诱导了休止期向生长期的转变。此外，FGF12 过表达调节了 PDGF-CC、MDK 和 HB-EGF 的表达，这些因子的处理表现出促进毛发生长的作用。总之，FGF12 通过诱导毛囊的生长期促进毛发生长，这表明其具有治疗脱发的潜力。
