Chen Tianming, Chen Ziming, Li Yuzhen, Zeng Bin, Zhang Zhe
Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-Vitro Diagnostic Reagents and Devices of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang 330013, China.
College of Pharmacy, Shenzhen Technology University, Shenzhen 518118, China.
J Fungi (Basel). 2022 Aug 22;8(8):885. doi: 10.3390/jof8080885.
Kojic acid is an important secondary metabolite of industrial importance produced by . The kojic acid gene cluster plays an essential role in kojic acid production, and harbors , and . The deletion of , encoding a major facilitator superfamily (MFS) transporter, did not completely abolish kojic acid production, implying that other transporters are required for the transport of kojic acid. The aim of this study is to look for the transporters involved in kojic acid production. Here, encoding a novel MFS transporter was identified, which was adjacent to in the kojic acid gene cluster. The deletion of contributed to the hyphal growth, conidial production and biomass of . Moreover, is required for heat- and cell-wall-stress tolerance but not oxidative and osmotic stress. The disruption of impaired kojic acid production with the reduced expression of , and . Furthermore, when was deleted in the -disrupted strain, the yield of kojic acid declined to the same level as that of the -deletion mutant, whereas the production of kojic acid was recovered when was overexpressed in the knockout strain, suggesting that acts downstream of . AoKap4 was the second identified MSF transporter involved in kojic acid production after was found a decade ago. This study contributes to a better understanding of the biological roles of the MFS transporter and lays a foundation for future studies on kojic acid synthesis in .
曲酸是由……产生的具有工业重要性的重要次生代谢产物。曲酸基因簇在曲酸生产中起关键作用，包含……、……和……。编码主要协助转运蛋白超家族（MFS）转运体的……缺失并未完全消除曲酸的产生，这意味着曲酸的转运还需要其他转运体。本研究的目的是寻找参与曲酸生产的转运体。在此，鉴定出了一个编码新型MFS转运体的……，它在曲酸基因簇中与……相邻。……的缺失促进了……的菌丝生长、分生孢子产生和生物量。此外，……是耐热和细胞壁应激耐受性所必需的，但不是氧化和渗透应激所必需的。……的破坏通过……、……和……表达的降低损害了曲酸的产生。此外，当在……破坏的菌株中缺失……时，曲酸产量下降到与……缺失突变体相同的水平，而当在……敲除菌株中过表达……时，曲酸的产生得以恢复，这表明……在……下游起作用。AoKap4是十年前发现……后第二个被鉴定出的参与曲酸生产的MSF转运体。本研究有助于更好地理解MFS转运体的生物学作用，并为未来……中曲酸合成的研究奠定基础。
