Bassiouni Mohamed, Arens Philipp, Zabaneh Samira Ira, Olze Heidi, Horst David, Roßner Florian
Department of Otorhinolaryngology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
Institute of Pathology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 10117 Berlin, Germany.
J Clin Med. 2022 Aug 18;11(16):4826. doi: 10.3390/jcm11164826.
The differential involvement of the macrophage activation phenotypes (M1 vs. M2) has been linked to disease severity in various chronic inflammatory disorders. Pharmacologic manipulation of the M1/M2 macrophage polarization has shown therapeutic potential. Cholesteatoma is a destructive chronic middle ear disease with potentially life-threatening complications. The distribution of macrophage polarization phenotypes in middle ear cholesteatoma has not been described. In the present study, human cholesteatoma specimens acquired during tympanomastoidectomy were retrospectively retrieved and immunohistochemically characterized using a combination of antibodies labeling M1 macrophages (CD80), M2 macrophages (CD163), and total macrophages (CD68). The correlations between the immunohistochemical findings and clinical presentation were assessed. The findings revealed that cholesteatomas with more extensive ossicular erosion demonstrated a significantly higher number of M1 (CD80+) cells and a higher M1/M2 ratio than less invasive cholesteatomas (Wilcoxon test, p < 0.05). The extent of ossicular erosion correlated significantly with the M1/M2 ratio (Spearman correlation coefficient ρ = 0.4, p < 0.05). Thus, the degree of ossicular erosion in human acquired cholesteatoma appears to be related to the M1/M2 macrophage polarization. The investigation of macrophage polarization and functions in various clinical presentations of middle ear cholesteatoma is of great interest since it may contribute to the development of pharmaceutical treatment approaches.
巨噬细胞激活表型（M1 与 M2）的不同参与情况已与多种慢性炎症性疾病的疾病严重程度相关联。对 M1/M2 巨噬细胞极化的药理学调控已显示出治疗潜力。胆脂瘤是一种具有潜在危及生命并发症的破坏性慢性中耳疾病。中耳胆脂瘤中巨噬细胞极化表型的分布尚未见描述。在本研究中，回顾性收集了鼓室乳突切开术中获取的人类胆脂瘤标本，并使用标记 M1 巨噬细胞（CD80）、M2 巨噬细胞（CD163）和总巨噬细胞（CD68）的抗体组合进行免疫组织化学特征分析。评估了免疫组织化学结果与临床表现之间的相关性。结果显示，与侵袭性较小的胆脂瘤相比，具有更广泛听骨侵蚀的胆脂瘤表现出显著更多的 M1（CD80+）细胞和更高的 M1/M2 比值（Wilcoxon 检验，p < 0.05）。听骨侵蚀程度与 M1/M2 比值显著相关（Spearman 相关系数 ρ = 0.4，p < 0.05）。因此，人类后天性胆脂瘤的听骨侵蚀程度似乎与 M1/M2 巨噬细胞极化有关。对中耳胆脂瘤各种临床表现中巨噬细胞极化及其功能的研究具有重要意义，因为它可能有助于药物治疗方法的开发。
