Promny Theresa, Kutz Chiara-Sophia, Jost Tina, Distel Luitpold V, Kadam Sheetal, Schmid Rafael, Arkudas Andreas, Horch Raymund E, Kengelbach-Weigand Annika
Department of Plastic and Hand Surgery, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
Department of Radiation Oncology, University Hospital Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany.
J Pers Med. 2022 Aug 5;12(8):1284. doi: 10.3390/jpm12081284.
The application of lipotransfer after breast-conserving therapy (BCT) and irradiation in breast cancer patients is an already widespread procedure for reconstructing volume deficits of the diseased breast. Nevertheless, the safety of lipotransfer has still not been clarified yet due to contradictory data. The goal of this in vitro study was to further elucidate the potential effects of lipotransfer on the irradiated remaining breast tissue. The mammary epithelial cell line MCF-10A was co-cultured with the fibroblast cell line MRC-5 and irradiated with 2 and 5 Gy. Afterwards, cells were treated with conditioned medium (CM) from adipose-derived stem cells (ADSC), and the effects on the cellular functions of MCF-10A cells and on gene expression at the mRNA level in MCF-10A and MRC-5 cells were analyzed. Treatment with ADSC CM stimulated transmigration and invasion and decreased the surviving fraction of MCF-10A cells. Further, the expression of cytokines, extracellular, and mesenchymal markers was enhanced in mammary epithelial cells. Only an effect of ADSC CM on irradiated fibroblasts could be observed. The present data suggest epithelial-mesenchymal transition-like changes in the epithelial mammary breast cell line. Thus, the benefits of lipotransfer after BCT should be critically weighed against its possible risks for the affected patients.
在乳腺癌患者的保乳治疗（BCT）和放疗后应用脂肪移植，是一种已广泛用于修复患侧乳房体积缺损的方法。然而，由于数据相互矛盾，脂肪移植的安全性仍未得到明确。这项体外研究的目的是进一步阐明脂肪移植对放疗后剩余乳腺组织的潜在影响。将乳腺上皮细胞系MCF-10A与成纤维细胞系MRC-5共培养，并用2 Gy和5 Gy进行照射。之后，用脂肪来源干细胞（ADSC）的条件培养基（CM）处理细胞，并分析其对MCF-10A细胞的细胞功能以及MCF-10A和MRC-5细胞中mRNA水平基因表达的影响。用ADSC CM处理可刺激迁移和侵袭，并降低MCF-10A细胞的存活分数。此外，乳腺上皮细胞中细胞因子、细胞外和间充质标志物的表达增强。仅观察到ADSC CM对受照射成纤维细胞有影响。目前的数据表明，乳腺上皮细胞系存在上皮-间质转化样变化。因此，对于受影响的患者，应严格权衡BCT后脂肪移植的益处及其可能的风险。
