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High performance liquid chromatography determination of cystathionase activity in human and rabbit lenses.

作者信息

Bergad P L, Rathbun W B

出版信息

Ophthalmic Res. 1986;18(6):343-8. doi: 10.1159/000265461.

DOI:10.1159/000265461
PMID:3601351
Abstract

Cystathionase activity has been demonstrated in human and rabbit lenses, using L-homoserine as substrate. The amount of activity found was quite low, 0.05 and 0.08 nmol of alpha-ketobutyrate formed/h/mg protein in human and rabbit lenses, respectively. The human lens cystathionase was found to have a pH maximum of 8.5 and a Km of 23.8 mM for L-homoserine. A sensitive assay, involving the use of high performance liquid chromatography, was developed to measure the small amounts of alpha-ketobutyrate produced.

摘要

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