Vuletin Selak Gabriela, Raboteg Božiković Marina, Abrouk Danis, Bolčić Marija, Žanić Katja, Perica Slavko, Normand Philippe, Pujic Petar
Institute for Adriatic Crops and Karst Reclamation, Put Duilova 11, 21000 Split, Croatia.
Centre of Excellence for Biodiversity and Molecular Plant Breeding (CoE CroP-BioDiv), Svetošimunska Cesta 25, 10000 Zagreb, Croatia.
Microorganisms. 2022 Jul 28;10(8):1529. doi: 10.3390/microorganisms10081529.
Two bacteria belonging to the and genera were isolated from olive knots. Both bacterial strains were omnipresent in this study's olive orchard with high susceptibility of the autochthonous olive genotypes indicating coevolution of bacteria with host plants. Genomes of two endemic bacteria show conserved core genomes and genome plasticity. The ST1 genome has conserved virulence-related genes including genes for quorum sensing, pilus, and flagella biosynthesis, two copies of indole acetic acid biosynthesis (IAA) operons, type I-VI secretions systems, and genes for alginate and levan biosynthesis. Development of knots depends only on the presence of the ST1 strain which then allows paga strain co-infection and cohabitation in developed knots. The two bacteria are sensitive to a large number of antimicrobials, antibiotics, HO, and Cu (II) salts that can be efficiently used in propagation of bacterial free olive cultivars.
从橄榄瘤中分离出了属于两个属的两种细菌。在本研究的橄榄园中，这两种细菌普遍存在，本地橄榄基因型对其高度敏感，表明细菌与寄主植物共同进化。两种地方细菌的基因组显示出保守的核心基因组和基因组可塑性。ST1基因组具有保守的毒力相关基因，包括群体感应、菌毛和鞭毛生物合成的基因、两份吲哚乙酸生物合成（IAA）操纵子、I型至VI型分泌系统，以及藻酸盐和果聚糖生物合成的基因。瘤的形成仅取决于ST1菌株的存在，该菌株随后允许paga菌株在已形成的瘤中共感染和共存。这两种细菌对大量抗菌剂、抗生素、过氧化氢和铜（II）盐敏感，这些物质可有效地用于无细菌橄榄品种的繁殖。
